Recombinant Murine VEGF120
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
VEGF was initially purified from media conditioned by normal bovine pituitary folliculo-stellate cells and by a variety of transformed cell lines as a mitogen specific for vascular endothelial cells. Three mouse cDNA clones, which arise through alternative splicing and which encode mature mouse monomeric VEGF having 120, 164, or 188, amino acids, respectively, have been identified. Two receptor tyrosine kinases (RTKs), Flt-1 and Flk-1 (the mouse homologue of human KDR), both members of the type III subclass of RTKs containing seven immunoglobulin-like repeats in their extracellular domains, have been shown to bind VEGF with high affinity. VEGF has been found to be a potent angiogenesis inducer in vivo.
| 目录编号 | 125-06 |
|---|---|
| 产品编码 | rmVEGF120 |
| 制造国家 | United States or China |
| 规格/数量 | 2ug, 10ug, 1mg |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后，请储存于 -20°C。复溶后，可在 2-8°C 保存最多一周。为获得最大稳定性，请分装并储存于 -20°C。避免反复冻融。 |
| 运输 | 干冰。 |
| Synonyms | VEGFA, VEGF-A, Vascular permeability factor (VPF), VEGF, Vegf188, Vegf164, Vegfa |
| AA Sequence | MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R |
| Source | Escherichia coli |
| Molecular Weight | Approximately 28.4 kDa disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains. |
| Purity | >96% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active. The ED50 is 2-4ng/ml, as determined by HUVEC cell proliferation. |
| Physical Appearance | White lyophilized powder. |
| Formulation | Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4. |
| Endotoxin | < 1EU/μg of growth factor as determined by LAL method. |
| Reconstitution | Reconstitute in sterile distilled water containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. |