Recombinant Murine Tumor Necrosis-alpha
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Tumor necrosis factor alpha (TNF-α) is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable . The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and cross reactivity between the two species.
| 目录编号 | 123-01 |
|---|---|
| 产品编码 | rmTNF-α |
| 制造国家 | United States or China |
| 规格/数量 | 5ug, 20ug, 1mg |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后，请储存于 -20°C。复溶后，可在 2-8°C 保存最多一周。为获得最大稳定性，请分装并储存于 -20°C。避免反复冻融。 |
| 运输 | 干冰。 |
| Synonyms | Cachectin, DIF, TNFA, TNFa, TNFSF2, TNF-alpha, APC1 protein |
| AA Sequence | MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQL VVPADGLYL VYSQVLFKGQ GCPDYVLLTH TVSRFAISYQ EKVNLLSAVK SPCPKDTPEG AELKPWYEPI YLGGVFQLEK GDQLSAEVNL PKYLDFAESG QVYFGVIAL |
| Source | Escherichia coli |
| Molecular Weight | Approximately 17.3 kDa, a soluble 157 amino acid protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein. |
| Purity | >95% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active. ED50 is < 0.1ng/ml, corresponding to a specific activity > 1 × 10^7 units/mg, as determined by murine L929 cell cytolysis in the presence of Actinomycin D. |
| Physical Appearance | White lyophilized powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.2. |
| Endotoxin | < 1EU/μg of growth factor as determined by LAL method. |
| Reconstitution | Reconstitute in sterile distilled water containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. |