Recombinant Murine Leukemia Inhibitory Factor
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo.
| 目录编号 | 123-07 |
|---|---|
| 制造国家 | 中国 |
| 产品编码 | rMuLIF |
| 规格/数量 | 5ug, 25ug, or 1mg |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后，请储存于 -20°C。复溶后，可在 2-8°C 保存最多一周。为获得最大稳定性，请分装并储存于 -20°C。避免反复冻融。 |
| 运输 | 干冰。 |
| Synonyms | D-FACTOR, differentiation-stimulating factor |
| AA Sequence | MSPLPITPVN ATCAIRHPCH GNLMNQIKNQ LAQLNGSANA LFISYYTAQG EPFPNNVEKL CAPNMTDFPS FHGNGTEKTK LVELYRMVAY LSASLTNITR DQKVLNPTAV SLQVKLNATI DVMRGLLSNV LCRLCNKYRV GHVDVPPVPD HSDKEAFQRK KLGCQLLGTY KQVISVVVQA F |
| Source | Escherichia coli |
| Molecular Weight | Approximately 20 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids. |
| Purity | >98% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Activity determined by its ability to induce differentiation of murine M1 myeloid leukemic cells. Minimum detectable concentration in assay is 0.5ng/mL, corresponding to specific activity > 1 x 10^8 units/ mg. |
| Physical Appearance | White lyophilized powder |
| Formulation | Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in 20mM PB, pH 7.4, with 0.02% TWEEN 20. |
| Endotoxin | < 1EU/μg of growth factor as determined by LAL method. |
| Reconstitution | Reconstitute in sterile distilled water containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. |