Recombinant Human Keratinocyte Growth Factor-2
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Keratinocyte Growth Factor-2 (KGF-2) was originally identified from rat embryos by homology-based polymerase chain reaction. Human and mouse KGF-2 were subsequently cloned. The human KGF-2 cDNA encodes a 208 amino acid residue protein with a hydrophobic amino-terminal signal peptide. Human KGF-2 shares approximately 92% and 95% amino acid sequence identity with mouse and rat KGF-2, respectively. Among the FGF family members, KGF-2 is most closely related to FGF-7. KGF-2 transcripts has been shown to be most abundant in the embryo and adult lungs. Recombinant KGF-2 preparations have been shown to be mitogenic for epithelial and epidermal cells but not fibroblasts. Based on its in vitro biological activities and in vivo expression pattern, KGF-2 has been proposed to play unique roles in the brain, in lung development, wound healing and limb bud formation.
| 目录编号 | 104-10 |
|---|---|
| 产品编码 | rhKGF-2 |
| 制造国家 | United States or China |
| 规格/数量 | 5ug, 25ug, or 1mg |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后，请储存于 -20°C。复溶后，可在 2-8°C 保存最多一周。为获得最大稳定性，请分装并储存于 -20°C。避免反复冻融。 |
| 运输 | 干冰。 |
| Synonyms | FGF-10, FGF10 |
| AA Sequence | MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS |
| Source | Escherichia coli |
| Molecular Weight | Approximately 19.3 kDa, 170 amino acid residues consisting of Methionine and the mature human KGF-2 (amino acid residues 40 – 208). |
| Purity | >96% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active. The ED50 is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 10^6 units/mg, as determined by proliferation of BaF3 cells expressing FGF receptors. |
| Physical Appearance | White lyophilized powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | < 1EU/μg of growth factor as determined by LAL method. |
| Reconstitution | Reconstitute in sterile distilled water containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. |