IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL-2Rα, 75 kDa IL-2Rβ, and 64 kDa common gamma chain γc/IL-2Rγ) that are present on the cell surface in varying preformed complexes. Mature human IL-2 shares 56 % and 66 % amino acid sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity.
IL-2 是一种强效的免疫调节淋巴因子，由 T 细胞在抗原或促分裂刺激下产生。它可由 CD4+ 和 CD8+ T 细胞、γδ T 细胞、B 细胞、树突状细胞以及嗜酸性粒细胞表达。IL-2/IL-2R 信号通路对于 T 细胞增殖及其他免疫应答中关键功能的维持是必需的。IL-2 受体由三种亚单位组成（55 kDa 的 IL-2Rα、75 kDa 的 IL-2Rβ 以及 64 kDa 的共用 γ 链 γc/IL-2Rγ），这些亚单位以不同的预形成复合体存在于细胞表面。成熟的人 IL-2 与小鼠和大鼠 IL-2 的氨基酸序列同源性分别为 56% 和 66%。人 IL-2 与小鼠 IL-2 具有跨物种活性。
| 目录编号 | 101-02 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | rhIL-2 |
| 规格/数量 | 10 μg, 100 μg, or 1 mg |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后，请保存在 -20℃。复溶后，可在 2–8℃ 下保存长达一周。为获得最佳稳定性，建议分装后保存在 -20℃。避免反复冻融。 |
| 运输 | 凝胶包。 |
| Synonyms | IL2, T-cell Growth Factor, TCGF, Aldesleukin |
| AA Sequence | SAPTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT |
| Source | Escherichia coli |
| Molecular Weight | 15.4 kDa, a single non-glycosylated polypeptide chain containing 134 amino acids |
| Purity | > 95% |
| Physical Appearance | White lyophilized powder. |
| Formulation | Lyophilized from a 0.2µm filtered concentrated (1mg/ml) solution in PBS, pH 7.4. |
| Endotoxin | <0.1 ng/μg of protein (<1 EU/μg) |
| Reconstitution | Reconstitute in sterile distilled water containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. |