Recombinant Human Fibroblast Growth Factor-19
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Fibroblast growth factor 19 (FGF-19) belongs to the large FGF family which has at least 23 members. However, FGF-19, along with FGF-21 and FGF-23, belongs to a subfamily of atypical FGFs where it lacks a conventional heparin-binding motif. Therefore, its signal transduction is mediated by the cofactor βKlotho when interacting with FGFRs 1c, 2c and 3c. Interestingly, FGF-19 can interact directly with FGFR4 in absence of βKlotho. FGF-19 regulates metabolic processes including bile acid, lipid and glucose homeostasis, as well as hepatocyte proliferation. Administration of recombinant FGF-19 to mice decreased cholesterol 7-alpha-hydroxylase (CYP7A1) expression, the rate limiting step in bile acid biosynthesis pathway in the liver.
| 目录编号 | 104-19 |
|---|---|
| 制造国家 | 中国 |
| 产品编码 | rhFGF-19 |
| 规格/数量 | 5ug, 25ug, 1mg |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后，请储存于 -20°C。复溶后，可在 2-8°C 保存最多一周。为获得最大稳定性，请分装并储存于 -20°C。避免反复冻融。 |
| 运输 | 干冰。 |
| Synonyms | FGF19 |
| AA Sequence | MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCARGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCAFEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPMVPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK |
| Source | Escherichia coli |
| Molecular Weight | Approximately 21.8 kDa, a single non-glycosylated polypeptide chain containing 195 amino acids. |
| Purity | >95% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active. The ED50 is 100- 150ng/ml, as determined by proliferation of BALB/c 3T3 cells. |
| Physical Appearance | White lyophilized powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | < 1EU/μg of growth factor as determined by LAL method. |
| Reconstitution | Reconstitute in sterile distilled water containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. |