Recombinant Human Betacellulin
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Betacellulin (BTC) is a member of the epidermal growth factor (EGF) family of cytokines and appears to play a role in the growth and differentiation of pancreatic beta cells. BTC and Activin A synergistically can convert AR42J rat pancreatic cells from amylase-secreting cells to insulin-producing cells. Human BTC is initially synthesized as a glycosylated transmembrane precursor protein, which is then proteolytically cleaved to generate its mature form. At the amino acid sequence level, human mature BTC protein exhibits 80% identity with mouse BTC protein. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in bodily fluids, such as in serum, milk, and colostrum.
| 目录编号 | 107-12 |
|---|---|
| 产品编码 | rhBTC |
| 制造国家 | United States or China |
| 规格/数量 | 5ug, 20ug, or 1mg |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后，请储存于 -20°C。复溶后，可在 2-8°C 保存最多一周。为获得最大稳定性，请分装并储存于 -20°C。避免反复冻融。 |
| 运输 | 干冰。 |
| Synonyms | Probetacellulin |
| AA Sequence | DGNSTRSPET NGLLCGDPEE NCAATTTQSK RKGHFSRCPK QYKHYCIKGR CRFVVAEQTP SCVCDEGYIG ARCERVDLFY |
| Source | Escherichia coli |
| Molecular Weight | Approximately 9 kDa monomeric protein containing 80 amino acids, comprising the mature EGF homologous portion of BTC. |
| Purity | >98% by SDS-PAGE and HPLC analyses. |
| Biological Activity | ED50 is <0.05ng/ml, corresponding to a specific activity of ? 2 x 10^7units/mg, as determined by the proliferation of murine Balb/3T3 cells |
| Physical Appearance | White lyophilized powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.4. |
| Endotoxin | < 1EU/μg of growth factor as determined by LAL method. |
| Reconstitution | Reconstitute in sterile distilled water containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. |