Ready-to-use 3D Human Calvarial Osteoblast Spheroid Kit

Bone is a living organ maintained by osteoblasts and osteoclasts. Osteoblasts, the bone-forming cells, secrete bone matrix such as type I collagen, while they remain exclusively as an organized tight epithelium to separate the bone extracellular matrix from general extracellular fluid. As matrix grow, osteoblasts are incorporated into the matrix as osteocytes, which communicate with each other and surface layer osteoblasts through cell processes that run within canaliculi in the matrix. The complex interactions between cell-cell and cell-ECM are critical for the process of bone formation and mineralization. Furthermore, these complex interactions are strictly dependent on the three-dimensional (3D) environment. To better approximate the in vivo condition, ScienCell has developed a highly innovative ready-to-use 3D osteoblast spheroid kit (SP3D-HCOS) in which cells maintain direct cell-cell and cell-ECM interactions in all three dimensions. With ScienCell’s Ready-to-use 3D spheroid kit, researchers can generate more physiologically relevant data from 3D spheroid culture in 24 hours after thawing, without encountering the long and complex workflow of 3D culture.