大鼠脾细胞

The spleen stores and purifies erythrocytes, metabolizes hemoglobin, and recycles iron. The spleen also provides a critical function for the immune system by mounting a primary immune response to antigens in the blood and synthesizing antibodies. Splenocytes are mononuclear cells derived from the spleen and include T-lymphocytes, B-lymphocytes, NK-cells, and NK Tcells. Primary rat splenocytes (RS) can be used to isolate CD4+ T-cells, CD8+ T-cells, and CD45R+ B cells.

RS from ScienCell Research Laboratories are isolated from normal adult rat spleen. RS are depleted of splenic macrophages, cryopreserved directly after isolation, and delivered frozen. Each vial contains at least 10 million cells in 1 ml volume. RS are quality control tested for viability. RS are negative for mycoplasma, bacteria, yeast, and fungi. RS can be maintained for a limited period of time in culture using the conditions provided by ScienCell Research Laboratories and are not intended for long-term culture.

Recommended Medium

It is recommended to use HematoGro Medium (HemGM, Cat. #5501) for short-term maintenance of RS in vitro.

脾脏具有储存和清除红细胞、代谢血红蛋白以及回收铁的重要功能。同时，脾脏在免疫系统中发挥关键作用，可对血液中的抗原产生初级免疫应答并合成抗体。脾细胞（Splenocytes）是来源于脾脏的单核细胞群体，包括T淋巴细胞、B淋巴细胞、NK细胞以及NKT细胞。原代大鼠脾细胞（RS）可用于分离CD4+ T细胞、CD8+ T细胞及CD45R+ B细胞。

ScienCell Research Laboratories 提供的大鼠脾细胞（RS）分离自正常成年大鼠脾脏。该细胞群体已去除脾巨噬细胞，在分离后立即进行冻存，并以冷冻形式提供。每管含有不少于1000万个细胞，体积为1 ml。RS经质量控制检测，具有良好的细胞活力，并确认不含支原体、细菌、酵母及真菌污染。RS可在ScienCell推荐的条件下进行短期体外培养，但不适用于长期培养。

推荐培养基

建议使用 HematoGro 培养基（HemGM，产品编号：5501）用于RS的短期体外维持培养。