大鼠唾液腺成纤维细胞

The salivary gland produces enzyme-containing saliva to lubricate the oral cavity and assist with digestion. It consists of three pairs of major salivary glands and many minor ones in the oral cavity. Histology studies show that the salivary gland is a compound tubuloalveolar gland, surrounded by connective tissue to further divide the gland into lobes and lobules. The secretory end pieces consist of a spherical mass of cells called acinus. Rat salivary gland fibroblasts (RSGF) are embryonic mesoderm derived mesenchymal cells and they are responsible for synthesis of extracellular matrix such as type I and/or type III collagen. RSGF are very versatile and provide a useful tool for studying collagenous fibroma and oral neoplasms.

RSGF from ScienCell Research Laboratories are isolated from postnatal day 2 rat salivary gland. RSGF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. RSGF characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. RSGF are negative for mycoplasma, bacteria, yeast, and fungi. RSGF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing RSGF in vitro.

唾液腺产生含有酶的唾液，用于润滑口腔并帮助消化。唾液腺由三对主要唾液腺以及分布于口腔内的许多小唾液腺组成。组织学研究表明，唾液腺属于复管泡状腺，周围由结缔组织包裹，并进一步将腺体分隔成腺叶和小叶。分泌末端由称为腺泡（acinus）的球状细胞团组成。大鼠唾液腺成纤维细胞（RSGF）是来源于胚胎中胚层的间充质细胞，负责合成I型和/或III型胶原蛋白等细胞外基质。RSGF具有很强的多功能性，可用于研究胶原性纤维瘤和口腔肿瘤。

ScienCell Research Laboratories提供的大鼠唾液腺成纤维细胞（RSGF）分离自出生后第2天（P2）的大鼠唾液腺。RSGF在第1代进行冻存，并以冷冻状态运输。每瓶含有超过5 × 10⁵个细胞，体积为1 ml。RSGF通过其梭形形态以及使用针对纤连蛋白的特异性抗体进行免疫荧光检测来鉴定。RSGF经检测不含支原体、细菌、酵母和真菌。在ScienCell Research Laboratories提供的培养条件下，RSGF可保证进一步扩增5个群体倍增。

推荐培养基

建议使用成纤维细胞培养基（Fibroblast Medium，FM，产品编号2301）对RSGF进行体外培养。

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