大鼠肺泡上皮细胞

Pulmonary alveolar epithelial cells, comprised of alveolar type I and type II epithelial cells, line more than 99% of the internal surface area of the lung. Type I cells are large squamous cells whose thin cytoplasmic extensions cover >95% of the internal lung surface. They contain aquaporins and exhibit the highest osmotic water permeability of any mammalian cell type. Type II cells, which cover 2-5% of the internal lung surface, produce, secrete, and recycle pulmonary surfactant. The currently accepted hypothesis is that Type II cells maintain pulmonary fluid homeostasis by regulating active Na+ transport in the lungs, whereas Type I cells are "inert" cells that provide only a barrier function. A recent study, however, suggests that Type I cells may also regulate ion and fluid transport.

RPAEpiC from ScienCell Research Laboratories are isolated from rat lung. RPAEpiC are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. RPAEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18. RPAEpiC are negative for, mycoplasma, bacteria, yeast, and fungi. RPAEpiC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories. This cell type, however, is not recommended for expanding or long term cultures since the cells would differentiate to type I alveolar epithelial cells immediately after plating and type I alveolar epithelial cells do not proliferate in culture.

Recommended Medium

It is recommended to use Alveolar Epithelial Cell Medium (AEpiCM, Cat. #3201) for culturing RPAEpiC in vitro.

肺泡上皮细胞由 I 型和 II 型肺泡上皮细胞组成，覆盖肺内部超过 99% 的表面积。I 型肺泡上皮细胞体积较大，呈鳞状，其纤薄的胞质延伸覆盖超过 95% 的肺内部表面积。I 型细胞含有水通道蛋白，并具有哺乳动物细胞中最高的渗透性水通透性。II 型肺泡上皮细胞覆盖肺内部约 2–5% 的表面积，负责肺表面活性物质的合成、分泌及再循环。目前普遍接受的观点认为，II 型细胞通过调节肺部 Na⁺ 的主动转运来维持肺液稳态，而 I 型细胞则主要作为发挥屏障功能的“惰性”细胞。然而，近期研究表明，I 型细胞可能同样参与离子和液体转运的调控。

ScienCell Research Laboratories 提供的大鼠肺泡上皮细胞（RPAEpiC）分离自大鼠肺组织。RPAEpiC 在 P0 代进行冻存，并以冷冻状态提供。每瓶含有 >1 × 10⁶ 个细胞，体积为 1 ml。RPAEpiC 采用针对细胞角蛋白-18（cytokeratin-18）的特异性抗体进行免疫荧光表征。RPAEpiC 的支原体、细菌、酵母及真菌检测结果均为阴性。根据 ScienCell Research Laboratories 提供的培养条件，RPAEpiC 可保证继续进行培养。但由于该细胞类型接种后会立即分化为 I 型肺泡上皮细胞，而 I 型肺泡上皮细胞在培养过程中不发生增殖，因此不建议将 RPAEpiC 用于细胞扩增或长期培养。

推荐培养基

推荐使用肺泡上皮细胞培养基（Alveolar Epithelial Cell Medium，AEpiCM，产品编号：3201）进行 RPAEpiC 的体外培养。

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