大鼠垂体细胞

The pituitary is a small, pea-sized gland located at the base of the brain. It is responsible for controlling and coordinating: 1) growth and development, 2) the function of various body organs, and 3) the function of other glandular organs. The study of pituitary gland development provides a remarkable example of cell specification. There are six major cell types of the anterior (endocrine) pituitary, and eight hormones: growth hormone, prolactin, thyrotrophin stimulating hormone, adrenocorticotrophic hormone, leutinizing hormone, follicle stimulating hormone, melanocyte stimulating hormone, and endorphin are produced by different pituitary cells, which are located in specific regions of the pituitary gland. Many pituitary cell types have been shown to express natriuretic peptide receptors and to respond to natriuretic peptides to stimulate cGMP accumulation.

RPC from ScienCell Research Laboratories are isolated from postnatal day 8 CD® IGS rats. RPC are cryopreserved after purification and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. RPC are negative for mycoplasma, bacteria, yeast and fungi. RPC are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories; however, RPC are not recommended for expanding or long-term cultures due to limited expansion capacity.

Recommended Medium

It is recommended to use Epithelial Cell Medium (EpiCM, Cat. #4101) for the culturing of RPC in vitro.

垂体是位于脑底部的一个豌豆大小的内分泌腺体，负责调控和协调：1）机体的生长与发育；2）多种器官的功能；以及3）其他内分泌腺体的活动。垂体发育研究为细胞命运决定和细胞分化提供了经典模型。垂体前叶（腺垂体）包含六种主要细胞类型，可分泌八种激素，包括生长激素（GH）、催乳素（PRL）、促甲状腺激素（TSH）、促肾上腺皮质激素（ACTH）、黄体生成素（LH）、卵泡刺激素（FSH）、黑色素细胞刺激素（MSH）和内啡肽（Endorphin）。这些激素由不同类型的垂体细胞产生，并分布于垂体的特定区域。研究表明，多种垂体细胞类型表达利钠肽受体，并可响应利钠肽刺激，促进环磷酸鸟苷（cGMP）的积累。

ScienCell研究实验室提供的大鼠垂体细胞（RPC）分离自出生后第8天（P8）的CD® IGS大鼠。RPC经纯化后进行冻存，并以冻存状态运输。每支冻存管含有 >5 × 10⁵ 个细胞，总体积为1 mL。RPC经检测确认无支原体、细菌、酵母和真菌污染。在ScienCell推荐的培养条件下，RPC可继续进行培养；但由于其增殖能力有限，不建议用于扩增培养或长期培养。

推荐培养基

建议使用上皮细胞培养基（Epithelial Cell Medium，EpiCM，产品编号 4101）进行RPC的体外培养。