大鼠中脑神经元

The midbrain plays a critical role in processing sensory information and regulating motor control and coordination. The midbrain is home to the colliculi, areas responsible for processing audio and visual information and the substantia nigra, a region involved in movement initiation. A progressive loss of substantia nigra neurons underlies Parkinson’s disease and other disruptions in midbrain function can result in disorders such as Parinaud’s syndrome. Neurons are anatomic, functional, and trophic units of the brain. Neurons are dynamically polarized cells responsible for electrochemically transmitting information throughout the nervous system. Despite great variability in size and shape, all neurons share common morphological features, the key elements of a highly complex communication network. In rat, the adult brain has been shown to contain around 200 million neurons. Primary rat neurons-midbrain (RN-mb) are a useful in vitro model for studying Parkinson’s disease and disorders associated with the midbrain.

RN-mb from ScienCell Research Laboratories are isolated from rat midbrain. RN-mb are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. RN-mb are characterized by immunofluorescence with antibodies specific to β-tubulin III. RN-mb are negative for mycoplasma, bacteria, yeast, and fungi. RN-mb are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, RN-mb are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.

Recommended Medium

It is recommended to use neuronal medium (NM, Cat. #1521) for culturing RN-mb in vitro.

中脑在感觉信息处理以及运动控制与协调中发挥关键作用。中脑包含上、下丘等负责处理听觉和视觉信息的结构，以及参与运动启动的黑质区域。黑质神经元的进行性丢失是帕金森病的重要病理基础，而其他中脑功能异常也可能导致如 Parinaud 综合征等疾病。

神经元是脑组织在解剖学、功能学和营养学上的基本单位。神经元是一类具有动态极性的细胞，负责在整个神经系统中通过电化学方式传递信息。尽管神经元在大小和形态上存在较大差异，但所有神经元都具有共同的形态学特征，是高度复杂神经通讯网络的重要组成部分。研究表明，成年大鼠脑内约含有 2 亿个神经元。原代大鼠中脑神经元（RN-mb）是研究帕金森病及其他中脑相关疾病的重要体外模型。

ScienCell Research Laboratories 提供的 RN-mb 分离自大鼠中脑组织。RN-mb 于 P0 代进行冻存，并以冷冻形式提供。每瓶含有超过 1 × 10⁶ 个细胞，总体积为 1 mL。RN-mb 采用 β-tubulin III 特异性抗体进行免疫荧光鉴定。经检测，RN-mb 不含支原体、细菌、酵母及真菌污染。

在 ScienCell Research Laboratories 提供的培养条件下，RN-mb 可保证后续培养；但由于该细胞在体外培养中不发生增殖，因此不建议用于扩增培养或长期培养。

推荐培养基

建议使用神经元培养基（NM，产品编号：#1521）进行 RN-mb 的体外培养。