大鼠骨髓间充质干细胞
大鼠骨髓间充质干细胞（RMSC-bm）分离自成年大鼠的骨髓。RMSC-bm 在第 1 代时进行冻存，并以冷冻状态运输。每瓶含有超过 5 × 105 个细胞，体积为 1 ml。
Mesenchymal stem cells (MSC) are a well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. The developmental plasticity of MSC has generated tremendous interest because of the potential use of mesenchymal stem cells in regenerative medicine to replace damaged tissues. MSC cultured without serum in the presence of transformation growth factors will differentiate into chondrocytes. In contrast, MSC cultured in serum with ascorbic acid and dexamethasone will differentiate into osteoblasts. With their renewal capability, MSC have the potential to be transplanted into an injured site or seeded on a biomimetic scaffold to generate appropriate tissue constructs.
RMSC-bm from ScienCell Research Laboratories are isolated from adult CD® IGS rat bone marrow. RMSC-bm are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. RMSC-bm are characterized by immunofluorescence with antibodies to CD73 and/or CD90, Oil Red O staining after adipogenic differentiation, and/or Alizarin Red staining after osteogenic differentiation. RMSC-bm are negative for mycoplasma, bacteria, yeast, and fungi. RMSC-bm are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. #7501) for culturing RMSC-bm in vitro.
间充质干细胞（MSC）是一类特征明确的成体干细胞群体。它们具有分化为成熟细胞的潜能，可产生脂肪、软骨、骨、肌腱和肌肉。MSC 的发育可塑性引起了广泛关注，这是因为间充质干细胞具有应用于再生医学、替代受损组织的潜力。在转化生长因子存在的无血清条件下培养 MSC，可使其分化为软骨细胞。相比之下，在含血清、抗坏血酸和地塞米松的条件下培养 MSC，可使其分化为成骨细胞。凭借其自我更新能力，MSC 有望被移植至受损部位，或接种于仿生支架上，以生成适宜的组织构建体。
ScienCell Research Laboratories 的大鼠骨髓间充质干细胞（RMSC-bm）分离自成年 CD® IGS 大鼠的骨髓。RMSC-bm 在第 1 代时进行冻存，并以冷冻状态运输。每瓶含有超过 5 × 105 个细胞，体积为 1 ml。RMSC-bm 采用针对 CD73 和/或 CD90 的特异性抗体进行免疫荧光鉴定，并通过成脂分化后的油红 O 染色和/或成骨分化后的茜素红染色进行鉴定。RMSC-bm 经检测不含支原体、细菌、酵母和真菌。在 ScienCell Research Laboratories 提供的培养条件下，RMSC-bm 保证可进一步培养。
推荐培养基
建议使用 Mesenchymal Stem Cell Medium（MSCM，产品编号 #7501）在体外培养 RMSC-bm。
| 目录编号 | R7500 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | RMSC-bm |
| 规格/数量 | 5 x 10^5 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后请立即将细胞从干冰直接转移至液氮中，并在进行细胞培养实验前始终保存在液氮中。 |
| 运输 | 干冰。 |
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