大鼠淋巴成纤维细胞

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen. There is evidence showing that fibroblasts in various organs are intrinsically different. In the lymphoid compartment, fibroblasts construct a unique conduit system with intriguing size exclusion which plays a fundamental role in regulating immune response and homeostasis of tissue fluids.

RLF from ScienCell Research Laboratories are isolated from rat lymph nodes. RLF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. RLF are characterized by their spindle-shaped morphology and immunofluorescence with antibody specific to fibronectin. RLF are negative for mycoplasma, bacteria, yeast, and fungi. RLF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing RLF in vitro.

成纤维细胞是来源于胚胎中胚层的间充质细胞。由于成纤维细胞是最容易在培养条件下生长的细胞类型之一，因此被广泛应用于各种细胞和分子研究。其较强的耐受性也使其适用于多种实验操作，包括基因转染和显微注射等研究。通常，成纤维细胞可分泌非刚性的细胞外基质，其中富含 I 型和/或 III 型胶原蛋白。有证据表明，不同器官中的成纤维细胞在内在特性上存在差异。在淋巴组织中，成纤维细胞可构建具有独特尺寸排阻特性的管道系统，在免疫反应和组织液稳态的调控中发挥重要作用。

ScienCell Research Laboratories 的大鼠淋巴成纤维细胞（RLF）分离自大鼠淋巴结。RLF 在第 1 代时进行冻存，并以冷冻状态运输。每瓶含有超过 5 × 10⁵ 个细胞，体积为 1 ml。RLF 可通过其梭形形态以及使用成纤维连接蛋白（fibronectin）特异性抗体进行免疫荧光鉴定。RLF 经检测不含支原体、细菌、酵母和真菌。在 ScienCell Research Laboratories 提供的培养条件下，RLF 保证可进一步扩增 5 个群体倍增。

推荐培养基

建议使用 Fibroblast Medium（FM，产品编号 #2301）在体外培养 RLF。

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