大鼠晶状体上皮细胞

The mammalian lens consists of two cell types, lens fiber cells which form the bulk of the lens, and a monolayer of epithelial cells that cover the anterior surface of the fibers. Lens epithelial cells are responsible for homeostasis regulation of the lens, including electrolyte and fluid transport. Under normal development, lens epithelial cells progressively differentiate and mature. Lens epithelial cells then migrate from the equatorial region into the interior of the lens to produce transparent crystallins, elongate to form lens fiber cells, and eventually lose their nuclei and other organelles. Studies have shown that lens epithelial cell differentiation and lens polarization are regulated by growth factors present in the ocular fluids, such as epidermal growth factor, basic fibroblast growth factor, insulin growth factor, and insulin.

RLEpiC from ScienCell Research Laboratories are isolated from the adult CD® IGS rat lens. RLEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. RLEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18. RLEpiC are negative for mycoplasma, bacteria, yeast and fungi. RLEpiC are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories; however, RLEpiC are not recommended for long-term cultures due to limited expansion capacity and senescence after subculturing.

Recommended Medium

It is recommended to use Epithelial Cell Medium-animal (EpiCM-a, Cat. #4131) for culturing RLEpiC in vitro.

哺乳动物的晶状体由两种细胞类型组成：构成晶状体主体的晶状体纤维细胞，以及覆盖在纤维细胞前表面的单层上皮细胞。晶状体上皮细胞在维持晶状体稳态方面发挥关键作用，包括电解质和液体的转运。在正常发育过程中，晶状体上皮细胞会逐步分化并成熟。随后，这些细胞从赤道区域向晶状体内部迁移，合成透明的晶状体蛋白（crystallins），并逐渐延长形成晶状体纤维细胞，最终失去细胞核及其他细胞器。研究表明，晶状体上皮细胞的分化及晶状体的极性受眼内液体中多种生长因子的调控，例如表皮生长因子、碱性成纤维细胞生长因子、胰岛素样生长因子以及胰岛素。

ScienCell Research Laboratories 提供的 RLEpiC（大鼠晶状体上皮细胞）分离自成年 CD® IGS 大鼠的晶状体。该细胞在第一代（P1）时进行冷冻保存，并以冻存形式提供。每个小瓶在 1 ml 体积中含有 >5 x 10⁵ 个细胞。RLEpiC 通过针对细胞角蛋白-18（cytokeratin-18）的抗体进行免疫荧光鉴定。经检测，RLEpiC 不含支原体、细菌、酵母和真菌。在 ScienCell Research Laboratories 提供的培养条件下，RLEpiC 可保证继续培养；但由于其扩增能力有限且在传代后易发生衰老，不建议进行长期培养。

推荐培养基

建议在体外培养 RLEpiC 时使用 Epithelial Cell Medium-animal（EpiCM-a，货号：4131）。