大鼠角膜基质细胞

The keratocytes, or corneal fibroblasts, are highly specialized cells that are sandwiched between orthogonally arranged layers of collagen lamellae in the corneal stroma. They play a key role in maintaining the structure and transparency of the cornea, as they are the source of stromal collagen and proglycans. They also play important roles in corneal wound healing and tissue repair, and are known to undergo phenotypic transformations in wounds due to the influence of growth factors and cytokines. Under normal conditions, the keratocytes in the adult cornea are relatively quiescent cells. In the event of corneal injury or trauma, however, the keratocytes differentiate into active, synthesizing cells and rapidly replace damaged stromal matrix. Cultured human keratocytes express functional IL-4Rs and IL-17R on the cell surface, suggesting that these cells may contribute to the role of IL-4 and IL-17 as mediators of allergic reactions in the cornea. Changes in gene expression were observed in keratocytes after interleukin-1 treatment, which provides important insight into gene expression and suggests novel therapeutic targets for the control of corneal inflammation.

RK from ScienCell Research Laboratories are isolated from CD® IGS rat cornea. RK are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. RK are characterized by fibroblast morphology and immunofluorescence with antibody specific to fibronectin. RK are negative for mycoplasma, bacteria, yeast and fungi. RK are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing RK in vitro.

角膜基质细胞（或称角膜成纤维细胞）是高度特化的细胞，位于角膜基质中呈正交排列的胶原层之间。它们在维持角膜结构和透明性方面发挥关键作用，因为它们是基质胶原和蛋白多糖的来源。角膜基质细胞还在角膜伤口愈合和组织修复中起重要作用，并且已知在伤口环境下会受到生长因子和细胞因子的影响而发生表型转化。在正常情况下，成年角膜中的角膜基质细胞属于相对静息状态的细胞。然而，在角膜受伤或创伤时，这些细胞会分化为活跃的合成细胞，并迅速替代受损的基质。体外培养的人角膜基质细胞在细胞表面表达功能性 IL-4R 和 IL-17R，提示这些细胞可能参与 IL-4 和 IL-17 在角膜过敏反应中的调控作用。对角膜基质细胞进行白细胞介素-1（IL-1）处理后观察到基因表达的变化，这为研究基因表达提供了重要信息，并提示了控制角膜炎症的新型治疗靶点。

ScienCell Research Laboratories 提供的 RK（大鼠角膜基质细胞）分离自 CD® IGS 大鼠角膜。RK 在第一代（P1）时进行冷冻保存，并以冻存形式提供。每瓶在 1 ml 体积中含有 >5 x 10⁵ 个细胞。RK 以成纤维细胞形态和针对纤维连接蛋白（fibronectin）的抗体免疫荧光鉴定。经检测，RK 不含支原体、细菌、酵母和真菌。在 ScienCell Research Laboratories 提供的培养条件下，RK 可保证进一步扩增 5 个细胞世代。

推荐培养基

建议在体外培养 RK 时使用 Fibroblast Medium（FM，货号：2301）。