大鼠肝星状细胞

Hepatic stellate cells (HSteC) are pericytes found in the perisinusoidal space presenting myofibroblast-like or lipocyte phenotypes. They participate in the homeostasis, repair, and regeneration of liver extracellular matrix, and control retinol metabolism, storage, and release. Following liver injury, HSteC proliferate, transform into myofibroblast-like cells, and produce type I collagen in the fibrotic liver. HSteC have been implicated as a regulator of hepatic microcirculation via cell contraction in the pathogenesis of intrahepatic portal hypertension. HSteC also possess voltage-activated calcium current, express the low affinity nerve growth factor receptor p75, and undergo apoptosis in response to nerve growth factor stimulation. These insights into the molecular regulation of HSteC activation will help lead to new therapeutic approaches in treatment of hepatic fibrosis by reducing morbidity and mortality in patients with chronic liver injury.

RHSteC from ScienCell Research Laboratories are isolated from postnatal day 2 or postnatal day 8 rat liver. RHSteC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. RHSteC are characterized by immunofluorescence with antibodies specific to desmin and/or smooth muscle actin. RHSteC are negative for mycoplasma, bacteria, yeast, and fungi. RHSteC are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended medium:

It is recommended to use Stellate Cell Medium (SteCM, Cat. #5301) for culturing RHSteC in vitro.

肝星状细胞（HSteC）是一类位于肝窦周隙（Disse 间隙）的周细胞（Pericytes），具有肌成纤维细胞样或脂细胞样表型。其参与肝脏细胞外基质的稳态维持、损伤修复及组织再生过程，并调控视黄醇（维生素 A）的代谢、储存和释放。肝脏损伤后，HSteC 会增殖并转化为肌成纤维细胞样细胞，在纤维化肝脏中产生 I 型胶原蛋白。研究表明，HSteC 可通过细胞收缩调节肝脏微循环，在肝内门静脉高压的发生发展过程中发挥重要作用。此外，HSteC 还具有电压依赖性钙离子电流，表达低亲和力神经生长因子受体 p75，并可在神经生长因子刺激下发生细胞凋亡。对 HSteC 活化分子调控机制的深入研究，有助于开发新的肝纤维化治疗策略，从而降低慢性肝损伤患者的发病率和死亡率。

ScienCell Research Laboratories 的大鼠肝星状细胞（RHSteC）分离自出生后第 2 天或第 8 天的大鼠肝脏组织。RHSteC 于第一代（P1）进行冻存，并以冻存状态交付。每支冻存管含 >5 x 10⁵ 个细胞，总体积为 1 mL。RHSteC 采用针对结蛋白（Desmin）和/或平滑肌肌动蛋白（Smooth Muscle Actin）的特异性抗体进行免疫荧光鉴定。经检测，RHSteC 不含支原体、细菌、酵母及真菌污染。在按照 ScienCell Research Laboratories 提供的培养条件进行培养的情况下，保证 RHSteC 可继续扩增至少 5 次群体倍增（Population Doublings）。

推荐培养基：

建议使用星状细胞培养基（Stellate Cell Medium，SteCM，产品编号：5301）进行 RHSteC 的体外培养。