成年大鼠肝星状细胞

Rat hepatic stellate cells (RHSteC) are pericytes found in the perisinusoidal space presenting myofibroblast-like or lipocyte phenotypes. RHSteC participate in the homeostasis, repair, and regeneration of liver extracellular matrix, and control retinol metabolism, storage, and release. Following liver injury, RHSteC proliferate, transform into myofibroblast-like cells, and produce type I collagen in the fibrotic liver. RHSteC have been implicated as a regulator of hepatic microcirculation via cell contraction in the pathogenesis of intrahepatic portal hypertension. RHSteC also possess voltage-activated calcium current, express the low affinity nerve growth factor receptor p75, and undergo apoptosis in response to nerve growth factor stimulation. These insights into the molecular regulation of RHSteC activation will help lead to new therapeutic approaches in treatment of hepatic fibrosis by reducing morbidity and mortality in patients with chronic liver injury.

RHSteC-a from ScienCell Research Laboratories are isolated from adult CD® IGS rat liver. RHSteC-a are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. RHSteC-a are characterized by immunofluorescence with antibodies specific to desmin and/or smooth muscle actin. RHSteC-a are negative for mycoplasma, bacteria, yeast, and fungi. RHSteC-a are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Stellate Cell Medium (SteCM, Cat. #5301) for culturing RHSteC-a in vitro.

大鼠肝星状细胞（RHSteC）是存在于窦周间隙中的周细胞，可呈现肌成纤维细胞样或脂细胞样表型。RHSteC 参与肝脏细胞外基质的稳态维持、修复和再生，并调控视黄醇的代谢、储存和释放。肝损伤发生后，RHSteC 会增殖并转化为肌成纤维细胞样细胞，在纤维化肝脏中产生 I 型胶原蛋白。RHSteC 可通过细胞收缩调节肝脏微循环，并参与肝内门静脉高压的发病机制。RHSteC 还具有电压激活型钙电流，表达低亲和力神经生长因子受体 p75，并可在神经生长因子刺激下发生凋亡。深入了解 RHSteC 激活的分子调控机制，将有助于通过降低慢性肝损伤患者的发病率和死亡率，为肝纤维化治疗的新方法提供依据。

ScienCell Research Laboratories 的成年大鼠肝星状细胞（RHSteC-a）分离自成年 CD® IGS 大鼠的肝脏。RHSteC-a 在第 1 代时进行冻存，并以冷冻状态运输。每瓶含有超过 5 × 10⁵ 个细胞，体积为 1 ml。RHSteC-a 采用针对结蛋白（desmin）和/或平滑肌肌动蛋白（smooth muscle actin）的特异性抗体进行免疫荧光鉴定。RHSteC-a 经检测不含支原体、细菌、酵母和真菌。在 ScienCell Research Laboratories 提供的培养条件下，RHSteC-a 保证可进一步扩增 5 个群体倍增。

推荐培养基
建议使用 Stellate Cell Medium（SteCM，产品编号 #5301）在体外培养 RHSteC-a。

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