Rat CRP ELISA Kit
ScienCell’s Rat CRP Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Rat Crp with a 96-well strip plate that is pre-coated with antibody specific for Crp.
C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.
ScienCell’s Rat CRP Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Rat Crp with a 96-well strip plate that is pre-coated with antibody specific for Crp. The detection antibody is a biotinylated antibody specific for Crp. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Crp with immunogen: Expression system for standard: NS0; Immunogen sequence: H20-S230. The kit is analytically validated with ready-to-use reagents.
| 目录编号 | EK0978 | ||||||||||||||||||||||||||||||||||||||||||
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| 制造国家 | 中国 | ||||||||||||||||||||||||||||||||||||||||||
| 产品编码 | Rat CRP-ELISA | ||||||||||||||||||||||||||||||||||||||||||
| 规格/数量 | 96 Tests in 8 x 12 divisible strips | ||||||||||||||||||||||||||||||||||||||||||
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 | ||||||||||||||||||||||||||||||||||||||||||
| 储存 | 可在4°C保存6个月，或在-20°C保存12个月。避免多次冻融循环。 | ||||||||||||||||||||||||||||||||||||||||||
| 运输 | 凝胶冰袋。 | ||||||||||||||||||||||||||||||||||||||||||
| Reactive Species | Rat | ||||||||||||||||||||||||||||||||||||||||||
| Application | ELISA | ||||||||||||||||||||||||||||||||||||||||||
| Sample Type | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). | ||||||||||||||||||||||||||||||||||||||||||
| Clonality Of Antibodies | polyclonal antibody from goat|polyclonal antibody from goat | ||||||||||||||||||||||||||||||||||||||||||
| Immunogen | Expression system for standard: NS0; Immunogen sequence: H20-S230 | ||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | <10 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
| Assay Range | 156 pg/ml - 10,000 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. | ||||||||||||||||||||||||||||||||||||||||||
| Kit Components | Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4 | ||||||||||||||||||||||||||||||||||||||||||
| Materials Required But Not Included In Kit | Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. | ||||||||||||||||||||||||||||||||||||||||||
| Sample Data |
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| Data Example Image |
Rat CRP PicoKine ELISA Kit standard curve
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| Intra Assay Consistency Inter Assay Consistency |
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| Reproducibility |
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| Gene Name | CRP | ||||||||||||||||||||||||||||||||||||||||||
| Uniport ID / Gene ID | P48199/25419 | ||||||||||||||||||||||||||||||||||||||||||