兔肾系膜细胞

Renal mesangial cells (RMC) are perivascular cells located within the central portion of the glomerular tuft between capillary loops, constituting 30-40% of the total glomerular cell population. RMC regulate the intraglomerular capillary flow and ultrafiltration surface via mesangial cell contraction and release of growth factors and vasoactive agents. By pinocytosis and phagocytosis, mesangial cells remove local accumulation of macromolecules in the mesangial space. Mesangial cells also synthesize, assemble and control turnover of the mesangial matrix. Overproduction of mesangial cells has been observed in various glomerular diseases, such as IgA nephropathy, mesangioproliferative glomerulonephritis, lupus nephritis, glomerulosclerosis and diabetic nephropathy. Such critical involvements suggest Rabbit RMC (RabRMC) are a useful model for studying mesangial injury and glomerular functions under both physiological and pathophysiological conditions.

RabRMC from ScienCell Research Laboratories are isolated from human renal tissue. RabRMC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. RabRMC are characterized by immunofluorescence with antibodies specific to fibronectin and/or smooth muscle actin. RabRMC are negative for mycoplasma, bacteria, yeast and fungi. RabRMC are guaranteed to further expand for 10 population doublings under the conditions specified by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Mesangial Cell Medium (MsCM, Cat. #4201) for the culturing of RabRMC in vitro.

肾系膜细胞（RMC）是位于肾小球毛细血管袢之间、肾小球毛细血管球中央区域的血管周围细胞，约占肾小球总细胞数的 30–40%。RMC 通过系膜细胞收缩以及生长因子和血管活性物质的释放，调控肾小球内毛细血管血流及超滤面积。同时，系膜细胞可通过胞饮和吞噬作用清除系膜间隙中局部积聚的大分子物质。此外，系膜细胞还参与系膜基质的合成、组装及更新调控。在多种肾小球疾病中，如 IgA 肾病、系膜增生性肾小球肾炎、狼疮性肾炎、肾小球硬化及糖尿病肾病等，均可观察到系膜细胞的异常增生。这些重要作用提示兔肾系膜细胞（RabRMC）是研究系膜损伤及肾小球生理与病理功能的有用模型。

RabRMC 由 ScienCell Research Laboratories 提供，分离自人肾组织。RabRMC 在第一代（P1）时进行冷冻保存，并以冻存状态交付。每瓶含有 >5 × 10⁵ 个细胞，体积为 1 ml。RabRMC 通过针对纤维连接蛋白（fibronectin）和/或 α-平滑肌肌动蛋白（smooth muscle actin）的特异性抗体进行免疫荧光鉴定。经检测，RabRMC 不含支原体、细菌、酵母及真菌污染。在 ScienCell Research Laboratories 提供的条件下，RabRMC 保证可进一步扩增至 10 代倍增。

推荐培养基

建议使用 Mesangial Cell Medium（MsCM，产品编号 #4201）进行 RabRMC 的 体外（in vitro） 培养。