嘌呤霉素（10 mg/ml；1 ml）

Puromycin is an aminonucleoside antibiotic isolated from Streptomyces alboniger. Puromycin terminates protein synthesis at the level of translation in prokaryotic and eukaryotic cells and is active against gram-positive bacteria and weakly active against gram-negative bacteria. Resistance to puromycin is conferred by the puromycin N-acetyl-transferase (pac) gene [1, 2]. Exogenous expression of the pac gene is commonly used in conjunction with puromycin to generate stable cell lines. Most working concentrations range from 1-10 μg/ml; however, the optimal concentration should be determined for individual cell types and culture conditions. In most cases, puromycin kills 99% of non-resistant cells within 48 hours.

嘌呤霉素是一种从白色链霉菌（Streptomyces alboniger）中分离获得的氨基核苷类抗生素。嘌呤霉素可在原核细胞和真核细胞的翻译水平终止蛋白质合成，对革兰氏阳性菌具有活性，对革兰氏阴性菌的活性较弱。嘌呤霉素 N-乙酰转移酶（pac）基因可赋予细胞对嘌呤霉素的抗性 [1, 2]。pac 基因的外源表达通常与嘌呤霉素联合使用，以构建稳定细胞系。

大多数情况下，嘌呤霉素的工作浓度范围为 1–10 μg/ml；但是，最佳浓度应根据具体细胞类型和培养条件分别确定。在大多数情况下，嘌呤霉素可在 48 小时内杀死 99% 的非耐药细胞。

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