C57BL/6 小鼠来源的神经束膜成纤维细胞

Perineurial fibroblasts are of mesenchymal origin and form the perineurium. The perineurium plays an important role in maintaining the integrity of the internal peripheral nerve environment by creating a physical barrier that, under physiologic conditions, limits the entry of biologically active proteins, infectious agents, and blood-borne cells into the nerve bundles. The perineurial fibroblasts are characterized by distinct ultrastructural features, including non-branching thin cytoplasmic processes coated by an external lamina and joined at their ends by a tight junction, few organelles, actin and vimentin filaments, and numerous pinocytotic vesicles. Perineurial fibroblasts are initially recruited from the surrounding mesenchyme to form a loose, permeable sheath around axons and Schwann cells, where they are separated by the extracellular matrix. These cells later undergo a mesenchymal-to-epithelial transition to form tight junctions and organize into the perineurium. Perineurial fibroblasts are immunoreactive for vimentin and epithelial membrane antigen but not for the Schwann cell marker S-100.

MPNF from ScienCell Research Laboratories are isolated from postnatal day 8 C57BL/6 mouse nerve. MPNF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. MPNF are characterized by immunofluorescence with antibodies specific to vimentin and/or fibronectin. MPNF are negative for mycoplasma, bacteria, yeast, and fungi. MPNF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing MPNF in vitro.

神经束膜成纤维细胞来源于间充质，并构成神经束膜。神经束膜通过形成一道物理屏障，在生理条件下限制生物活性蛋白、感染性因子以及血源性细胞进入神经束，从而在维持外周神经内部微环境的稳定性方面发挥关键作用。神经束膜成纤维细胞具有独特的超微结构特征，包括：不分支的细长细胞质突起，其表面覆盖外基膜，并在末端通过紧密连接相互连接；细胞器较少；含有肌动蛋白和波形蛋白丝；以及大量胞饮小泡。该类细胞最初由周围间充质募集而来，在轴突和施旺细胞周围形成一层松散且具有一定通透性的包裹结构，此时细胞之间由细胞外基质分隔。随后，这些细胞经历间充质向上皮样细胞的转化（mesenchymal-to-epithelial transition），逐渐形成紧密连接，并最终组织为结构完整的神经束膜。神经束膜成纤维细胞对波形蛋白（vimentin）和上皮膜抗原（epithelial membrane antigen）呈免疫反应阳性，而对施旺细胞标志物 S-100 呈阴性。

产品信息
ScienCell Research Laboratories 提供的小鼠神经束膜成纤维细胞（MPNF）分离自出生后第8天的 C57BL/6 小鼠神经组织。MPNF 在第一代传代时进行冻存，并以冻存形式运输。每瓶含有超过 5 × 10⁵ 个细胞，体积为 1 ml。MPNF 通过针对波形蛋白（vimentin）和/或纤维连接蛋白（fibronectin）的特异性抗体进行免疫荧光鉴定。该细胞产品经检测不含支原体、细菌、酵母和真菌。在 ScienCell 提供的培养条件下，MPNF 保证可继续扩增至少 5 个群体倍增。

推荐培养基
建议在体外培养 MPNF 时使用成纤维细胞培养基（Fibroblast Medium, FM，产品编号：2301）。