CD1 小鼠来源的神经束膜成纤维细胞

Perineurial fibroblasts are of mesenchymal origin and form the perineurium. The perineurium plays an important role in maintaining the integrity of the internal peripheral nerve environment by creating a physical barrier that, under physiologic condition, limits the entry of biologically active proteins, infectious agents, and blood-borne cells into the nerve bundles. The perineurial fibroblasts are characterized by distinct ultrastructural features, including non-branching thin cytoplasmic processes coated by an external lamina and joined at their ends by a tight junction, few organelles, actin and vimentin filaments, and numerous pinocytotic vesicles. Perineurial fibroblasts are initially recruited from the surrounding mesenchyme to form a loose, permeable sheath around axons and Schwann cells, where they are separated by the extracellular matrix. These cells later undergo a mesenchymal-to-epithelial transition to form tight junctions and organize into the perineurium. Perineurial fibroblasts are immunoreactive for vimentin and epithelial membrane antigen but not for the Schwann cell marker S-100.

MPNF from ScienCell Research Laboratories are isolated from postnatal day 8 CD-1 mouse nerve. MPNF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. MPNF are characterized by immunofluorescence with antibodies specific to vimentin and/or fibronectin. MPNF are negative for mycoplasma, bacteria, yeast, and fungi. MPNF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing MPNF in vitro.

神经束膜成纤维细胞属于间充质来源，形成神经束膜（perineurium）。神经束膜在维持外周神经内部微环境完整性方面起重要作用，可形成物理屏障，在生理条件下限制生物活性蛋白、感染性病原体以及血液来源细胞进入神经束内。神经束膜成纤维细胞具有典型的超微结构特征，包括无分支的细长胞质突起，其外表覆盖基底层，并在末端通过紧密连接相互连接；细胞内细胞器较少，含有肌动蛋白和波形蛋白丝，并具有大量胞饮小泡。

神经束膜成纤维细胞最初由周围间充质募集形成，在轴突与施旺细胞周围构建疏松且可通透的包被结构，各细胞之间由细胞外基质分隔。随后，这些细胞发生间充质-上皮样转变，形成紧密连接并组织为成熟的神经束膜结构。该类细胞对波形蛋白（vimentin）及上皮膜抗原（EMA）呈阳性表达，但不表达施旺细胞标志物 S-100。

ScienCell Research Laboratories 提供的 MPNF 分离自出生后第8天的 CD-1 小鼠神经组织。MPNF 在第1代（P1）进行冻存，并以冷冻形式交付。每支冻存管体积为 1 ml，细胞数量不少于 5 × 10⁵ 个。MPNF 通过针对波形蛋白和/或纤维连接蛋白（fibronectin）的特异性抗体进行免疫荧光鉴定。经检测，MPNF 不含支原体、细菌、酵母及真菌污染。在 ScienCell Research Laboratories 提供的培养条件下，MPNF 可保证至少进行 5 次群体倍增。

推荐培养基

建议在体外培养 MPNF 时使用成纤维细胞培养基（Fibroblast Medium，FM，产品编号 #2301）。