Mouse P-Cadherin ELISA Kit

P-Cadherin, also known as Cadherin-3 (CDH3), is a protein that in humans is encoded by the CDH3 gene. This gene is a classical cadherin from the cadherin superfamily. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16. It is involved in the loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis and juvenile macular dystrophy. Cells expressing CDH3 also adhere to one another more tightly than the parental cell line.

ScienCell's mouse P-Cadherin ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse P-Cadherin-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The mouse-specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of mouse P-Cadherin in the sample that is captured on the strips.