小鼠晶状体上皮细胞

The lens is a transparent structure in the eye that is designed to refract light and focus the light onto the retina. The mammalian lens consists of two cell types, lens fiber cells which form the bulk of the lens, and a monolayer of epithelial cells that cover the anterior surface of the fibers. Lens epithelial cells (LEpiC) are responsible for homeostasis regulation of the lens, including electrolyte and fluid transport. Under normal development, LEpiC progressively differentiate and mature. LEpiC then migrate from the equatorial region into the interior of the lens to produce transparent crystallins, elongate to form lens fiber cells, and eventually lose their nuclei and other organelles. Studies have shown that LEpiC differentiation and lens polarization are regulated by growth factors present in the ocular fluids, such as epidermal growth factor, basic fibroblast growth factor, insulin growth factor, and insulin. Mouse LEpiC (MLEpiC) are a useful model for studying issues associated with the lens, including cataracts.

MLEpiC from ScienCell Research Laboratories are isolated from postnatal day 8 CD-1 mouse lens. MLEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. MLEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18. MLEpiC are negative for mycoplasma, bacteria, yeast and fungi. MLEpiC are guaranteed to further expand for 5 population doublings.

Recommended Medium

It is recommended to use Epithelial Cell Medium (EpiCM Cat. #4101) for culturing MLEpiC in vitro.

晶状体是眼睛中的透明结构，主要功能是折射光线并将光聚焦到视网膜上。哺乳动物晶状体由两种细胞类型组成：构成晶状体主体的晶状体纤维细胞，以及覆盖在纤维细胞前表面的单层上皮细胞。晶状体上皮细胞（LEpiC）负责维持晶状体的稳态，包括电解质和液体的转运。在正常发育过程中，LEpiC 会逐步分化并成熟。随后，这些细胞从赤道区域迁移到晶状体内部，合成透明的晶状体蛋白（crystallins），延长形成晶状体纤维细胞，并最终失去细胞核及其他细胞器。研究表明，LEpiC 的分化及晶状体极性受眼内液体中多种生长因子的调控，例如表皮生长因子、碱性成纤维细胞生长因子、胰岛素样生长因子以及胰岛素。小鼠晶状体上皮细胞（MLEpiC）是研究晶状体相关问题（如白内障）的有用模型。

ScienCell Research Laboratories 提供的 MLEpiC 分离自出生后第 8 天的 CD-1 小鼠晶状体。MLEpiC 在第一代（P1）时进行冷冻保存，并以冻存形式提供。每瓶在 1 ml 体积中含有 >5 x 10⁵ 个细胞。MLEpiC 通过针对细胞角蛋白-18（cytokeratin-18）的抗体进行免疫荧光鉴定。经检测，MLEpiC 不含支原体、细菌、酵母和真菌。在 ScienCell Research Laboratories 提供的培养条件下，MLEpiC 可保证进一步扩增 5 个细胞世代。

推荐培养基

建议在体外培养 MLEpiC 时使用 Epithelial Cell Medium（EpiCM，货号：4101）。