C57BL/6 小鼠肝星状细胞

Hepatic stellate cells (HSteC) are pericytes found in the perisinusoidal space presenting myofibroblast-like or lipocyte phenotypes. They participate in the homeostasis, repair, and regeneration of liver extracellular matrix, and control retinol metabolism, storage, and release. Following liver injury, HSteC proliferate, transform into myofibroblast-like cells, and produce type I collagen in the fibrotic liver. HSteC have been implicated as a regulator of hepatic microcirculation via cell contraction in the pathogenesis of intrahepatic portal hypertension. HSteC also possess voltage-activated calcium current, express the low affinity nerve growth factor receptor p75, and undergo apoptosis in response to nerve growth factor stimulation. These insights into the molecular regulation of HSteC activation will help lead to new therapeutic approaches in treatment of hepatic fibrosis by reducing morbidity and mortality in patients with chronic liver injury.

MHSteC from ScienCell Research Laboratories are isolated from postnatal day 2 or postnatal day 8 C57BL/6 mouse liver. MHSteC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. MHSteC are characterized by immunofluorescence with antibodies specific to desmin and/or α-actin. MHSteC are negative for mycoplasma, bacteria, yeast, and fungi. MHSteC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, MHSteC are not recommended for long-term cultures due to limited expansion capacity and senescence after subculturing.

Recommended Medium

It is recommended to use Stellate Cell Medium (SteCM, Cat. #5301) for culturing MHSteC in vitro.

肝星状细胞（HSteC）是位于肝窦周隙（perisinusoidal space）的周细胞，可呈现肌成纤维细胞样或脂细胞样表型。其参与肝脏细胞外基质的稳态维持、组织修复与再生，并调控视黄醇的代谢、储存与释放。在肝损伤发生后，HSteC 增殖并转化为肌成纤维细胞样细胞，在纤维化肝脏中产生Ⅰ型胶原蛋白。研究表明，HSteC 可通过细胞收缩调节肝脏微循环，在肝内门静脉高压的发生发展过程中发挥重要作用。此外，HSteC 还具有电压依赖性钙电流，表达低亲和力神经生长因子受体 p75，并可在神经生长因子刺激下发生凋亡。对 HSteC 活化分子调控机制的深入研究，有助于开发肝纤维化治疗新策略，从而降低慢性肝损伤患者的发病率与死亡率。

ScienCell Research Laboratories 的小鼠肝星状细胞（MHSteC）分离自出生后第 2 天或第 8 天 C57BL/6 小鼠肝脏组织。MHSteC 于第一代（P1）进行冻存，并以冻存状态交付。每支冻存管含 >5 x 10⁵ 个细胞，总体积为 1 mL。MHSteC 经特异性抗 Desmin 和/或 α-Actin 抗体免疫荧光染色鉴定。MHSteC 经检测不含支原体、细菌、酵母及真菌污染。MHSteC 在 ScienCell Research Laboratories 提供的培养条件下可继续培养；但由于其增殖能力有限且传代后易发生细胞衰老，因此不建议长期培养。

推荐培养基

建议使用星状细胞培养基（Stellate Cell Medium，SteCM，产品编号：5301）进行 MHSteC 的体外培养。