经丝裂霉素 C 处理的来源于 CD-1 小鼠胚胎的成纤维细胞

Mouse Embryonic Fibroblasts (MEF) are used to support the growth of mouse and human pluripotent stem cells. MEF not only provide a substrate for pluripotent stem cells to grow on, but also secrete critical growth factors to maintain stem cell pluripotency. MEF are isolated from mouse embryos and used at early passages. To serve as feeder cells, MEF must be treated with mitomycin C or by irradiation to prevent cell proliferation. The treated cells can also be used to generate conditioned medium for feeder-free culture of pluripotent stem cells.

MEF-mt from ScienCell Research Laboratories are isolated from embryonic day 14 CD-1 mouse embryos. These cells have been treated with mitomycin C to prevent further cell division. They are cryopreserved at passage 3 and delivered frozen. Each vial contains 1 x 10⁶ cells in 1 ml volume. MEF-mt are characterized by immunofluorescence with antibody specific to fibronectin. MEF-mt are negative for mycoplasma, bacteria, yeast, and fungi. MEF-mt are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, MEF-mt are not recommended for expanding as mitomycin C treatment prevents further cell proliferation.

Recommended Medium

It is recommended to use DMEM (Cat. #09221) supplemented with 10% fetal bovine serum (FBS, Cat. #0010, 0025, 0500) for culturing MEF-mt in vitro.

小鼠胚胎成纤维细胞（MEF）可用于支持小鼠和人多能干细胞的生长。MEF 不仅可为多能干细胞提供生长所需的细胞基质，还可分泌维持干细胞多能性所必需的关键生长因子。MEF 分离自小鼠胚胎，并在早期传代阶段使用。作为饲养层细胞，MEF 必须经丝裂霉素 C 处理或辐照，以抑制细胞增殖。经处理的 MEF 还可用于制备条件培养基，以支持多能干细胞的无饲养层培养。

ScienCell Research Laboratories 提供的经丝裂霉素 C 处理的小鼠胚胎成纤维细胞（MEF-mt）分离自胚胎第 14 天的 CD-1 小鼠胚胎。细胞经丝裂霉素 C 处理，以抑制其进一步分裂。在第 3 代（P3）进行冻存，并以冷冻状态提供。每瓶含有 1 × 10⁶ 个细胞，体积为 1 ml。MEF-mt 采用针对纤连蛋白（fibronectin）的特异性抗体进行免疫荧光表征。MEF-mt 的支原体、细菌、酵母及真菌检测结果均为阴性。根据 ScienCell Research Laboratories 提供的培养条件，MEF-mt 可保证继续进行培养；但由于丝裂霉素 C 处理会抑制细胞进一步增殖，因此不建议用于细胞扩增。

推荐培养基

推荐使用 DMEM（产品编号：09221）并补充 10% 胎牛血清（FBS，产品编号：0010、0025 或 0500）进行 MEF-mt 的体外培养。

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