DMEM with High-Glucose, L-Glutamine and Sodium Pyruvate

Dulbecco's Modified Eagle's Medium DMEM with High-Glucose, L-Glutamine and Sodium Pyruvate (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins, as well as additional supplementary components. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Subsequently, DMEM has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 25 mM. For research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.