Mouse Embryonic Fibroblast-Conditioned Medium
MEF-cm is a liquid medium prepared from primary CF1 mouse embryonic fibroblasts treated with mitomycin C. The medium is sterile filtered and consists of one 100-mL bottle.
This product requires a minimum purchase of 10 bottles and a 3 week estimated time.
Please contact sales@sciencellonline.com to place an order.
Mouse Embryonic Fibroblast-Conditioned Medium (MEF-cm) is a serum free medium for the feeder independent culture of human pluripotent stem cells (hPSCs), including embryonic stem cells and induced pluripotent stem cells. MEF-cm is an efficient alternative to MEF feeder cells, supporting the self-renewal of hPSC and maintaining pluripotency in the presence of basic FGF and hPSC qualified matrix (e.g., BD MatrigelTM matrix). ScienCell's MEF-cm was prepared using high quality mitomycin C treated MEF (MEF-mt, Cat. #M7570-mt) derived from E13 embryos of CF1 mice. Serum free medium was conditioned with mitomycin C treated CF1 MEFs for 24 hours. The conditioned medium was collected daily for 7 days, pooled and sterilized by filtering through a 0.22 μm filter. Before using MEF-cm for hPSC culture, please add fresh recombinant human basic FGF (4 ng/mL) (rhbFGF, Cat #104-02) into the medium to make the complete medium. The complete MEF-cm has been tested for its ability to support hPSC self-renewal on BD MatrigelTM matrix in vitro. The medium color of each lot may vary but does not affect performance.
| 目录编号 | 5881 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | MEF-cm |
| 规格/数量 | 100 ml |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | MEF-cm 请存放于 ≤ -20°C 的手动除霜冰箱中。请在 2-8°C 黑暗条件下过夜解冻。未使用部分建议分装并存放于 ≤ -20°C。2-8°C 下长期保存（超过2周）不建议。避免反复冻融。 |
| 运输 | 干冰。 |
