C57BL/6 小鼠小脑星形胶质细胞

Astrocytes are the major cell type in the mammalian brain. They provide a variety of supportive functions to their partner neurons in the central nervous system (CNS), such as neuronal guidance during development, nutritional and metabolic support throughout life. Astrocytes have also been implicated in various pathological processes. Impairment of normal astrocyte functions during stroke and other insults can critically influence neuron survival. Long-term recovery after brain injury, through neurite outgrowth, synaptic plasticity, or neuron regeneration, is also influenced by astrocyte surface molecule expression and trophic factor release. Numerous studies have demonstrated that astrocytes are among the most functionally diverse group of cells in the CNS. Much of what we have learned about astrocytes is from in vitro studies and astrocyte culture is a useful tool for exploring the diverse properties of this cell type.

MA-c from ScienCell Research Laboratories are isolated from postnatal day 8 C57BL/6 mouse cerebellum. MA-c are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. MA-c are characterized by immunofluorescence with antibody specific to GFAP. MA-c are negative for mycoplasma, bacteria, yeast, and fungi. MA-c are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Astrocyte Medium-animal (AM-a, Cat. #1831) for culturing MA-c in vitro.

星形胶质细胞是哺乳动物脑内的重要细胞类型，在中枢神经系统（CNS）中发挥多种支持作用，包括在神经系统发育过程中引导神经元，以及在整个生命过程中为神经元提供营养和代谢支持。星形胶质细胞还参与多种病理生理过程。在卒中及其他脑损伤发生后，星形胶质细胞正常功能受损可能对神经元存活产生重要影响。此外，脑损伤后的长期恢复过程，包括神经突生长、突触可塑性变化及神经元再生，也会受到星形胶质细胞表面分子表达和营养因子释放的影响。大量研究表明，星形胶质细胞是中枢神经系统中功能多样性极高的细胞群体之一。我们对星形胶质细胞的许多认识来源于体外研究，而星形胶质细胞培养是研究其多种生物学特性和功能的重要实验手段。 ScienCell Research Laboratories 提供的小鼠小脑星形胶质细胞（MA-c）分离自出生后第 8 天的 C57BL/6 小鼠小脑。细胞以 P0 代冻存，并以冷冻状态运输。每瓶含有超过 5 x 10⁵ 个细胞，悬液体积为 1 mL。MA-c 采用针对胶质纤维酸性蛋白（GFAP）的特异性抗体进行免疫荧光鉴定。经检测，细胞不含支原体、细菌、酵母及真菌。在 ScienCell Research Laboratories 提供的培养条件下，MA-c 可进一步扩增至少 5 个群体倍增。 **推荐培养基** 建议使用动物源星形胶质细胞培养基（Astrocyte Medium-animal，AM-a，产品编号：1831）进行 MA-c 的体外培养。

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