猴痘病毒多重 qPCR 检测试剂盒

Monkeypox virus (MPXV) is a zoonotic virus that causes monkeypox in humans and animals. The enveloped double-stranded DNA virus belongs to the Orthopoxvirus genus in the Poxviridae family with a genome size of around 190 kb. The Poxviridae family also includes vaccinia virus, variola virus, cowpox virus, and several animal-related poxviruses. There are two distinct genetic clades of MPXV: the Central African clade and the West African clade. The Central African clade is thought to be more transmissible and causes more severe disease.

ScienCell's Monkeypox Virus Multiplex Detection Kit (MPXVD) is designed to detect the presence of monkeypox virus in dry swab specimen samples or extracted DNA samples from wet swab specimen, cell lysate, body fluid and other biological specimen samples. The cell lysis buffer and enhancer (Cat #GQ400a and GQ400b) are included in the kit to extract DNA from dry swab specimen samples for direct qPCR. The multiplex primer/probe set component (Cat #7178MPP) contains 3 primer/probe sets: MP1-FAM, MP2-FAM, and HR-HEX. Among them, MP1- FAM and MP2-FAM target highly conserved regions of monkeypox viruses. The carefully designed MP1-FAM and MP2-FAM primer/probe set can specifically recognize both the Central African clade and the West African clade of MPXV, but will not interact with other Poxviridae family viruses, including vaccinia virus, variola virus, and cowpox virus. HR-HEX primer/probe set targets human B2M gene and serves as a control to assess specimen quality. In addition, a non-infectious positive control (Cat #7178-Pos) and nuclease-free water (Cat #MB6048b-1) are included in the kit. The positive control (Cat #7178-Pos) consists of non-infectious viral DNA fragments spiked into human dermal fibroblast cells and serves to ensure reagents and instruments are working properly. Please refer to Tables 4 and 5 for results interpretation.

猴痘病毒（MPXV）是一种人畜共患病毒，可在人类和动物中引起猴痘。该病毒为有包膜的双链 DNA 病毒，属于痘病毒科（Poxviridae）正痘病毒属（Orthopoxvirus），基因组大小约为 190 kb。痘病毒科还包括牛痘病毒、天花病毒、痘苗病毒以及多种动物相关痘病毒。MPXV 存在两个不同的遗传分支：中非分支和西非分支，其中中非分支被认为具有更强的传播能力并可导致更严重的疾病。

ScienCell 的猴痘病毒多重 qPCR 检测试剂盒（MPXVD）旨在检测干拭子样本或从湿拭子样本、细胞裂解液、体液及其他生物样本中提取的 DNA 中是否存在猴痘病毒。试剂盒中包含细胞裂解缓冲液及其增强剂（货号 #GQ400a 和 GQ400b），用于从干拭子样本中提取 DNA，以便直接进行 qPCR 检测。多重引物/探针组分（货号 #7178MPP）包含三套引物/探针：MP1-FAM、MP2-FAM 和 HR-HEX。其中，MP1-FAM 和 MP2-FAM 靶向猴痘病毒的高度保守区域。经过精心设计，这两套引物/探针可特异性识别中非分支和西非分支的 MPXV，但不会与痘病毒科中的其他病毒（包括痘苗病毒、天花病毒和牛痘病毒）发生交叉反应。HR-HEX 引物/探针靶向人 B2M 基因，用作内参对照以评估样本质量。

此外，试剂盒中还包含非感染性阳性对照（货号 #7178-Pos）和无核酸酶水（货号 #MB6048b-1）。阳性对照（货号 #7178-Pos）由掺入人真皮成纤维细胞中的非感染性病毒 DNA 片段组成，用于确保试剂和仪器正常运行。结果判读请参见表 4 和表 5。