Malachite Green Phosphate Assay

Phosphorylation and dephosphorylation, which are the addition and removal of phosphate (PO43-) group to and from protein molecules respectively, are important regulatory mechanisms involved in cell-cycle regulation and signal transduction. ScienCell Malachite Green Phosphate Assay provides a simple colorimetric method for the determination of inorganic, soluble phosphate concentrations based on the complexation of malachite green oxalate with phoshomolybdate under acidic conditions. Applications of the kit include measurement of phosphate release, quantification of protein- or lipid- phosphorylation and etc. For quantification of protein or lipid bound phosphate, the phosphorylated proteins or lipids need to be hydrolyzed and neutralized before phosphate measurement are performed.