L-Lactate Assay
Monitoring lactate levels is an adequate indicator of the balance between tissue oxygen demand and utilization, and is useful when studying cellular physiology.
Lactate is an important intermediary in glucose metabolism. Under hypoxic or anaerobic conditions, lactate dehydrogenase (LDH) converts pyruvate, the final product of glycolysis, to lactate. Monitoring lactate levels is an adequate indicator of the balance between tissue oxygen demand and utilization, and is useful when studying cellular physiology. This colorimetric assay is based on the reduction of the tetrazolium salt, INT, in a NADH-coupled enzyme reaction to formazan, which exhibits an absorbance maximum at 490 nm. The intensity of the optical density is proportional to the lactate concentration.
| 目录编号 | 8308 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | LAC |
| 规格/数量 | 100 Tests in 96-well plate |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后，请将 Assay Buffer（#8308a）和 Reconstitution Solution（#8308e）存放于 4°C；L-乳酸标准品（#8308b）、酶混合物（#8308c）和底物混合物（#8308d）存放于 -20°C。 |
| 运输 | 干冰。 |