The delivery of foreign DNA into eukaryotic cells is one of the most common molecular biology techniques used to study biological mechanisms. However, unlike transformed cell lines, efficient transfection of primary cells can be challenging. KeraFectagen is a cationic polymer-based transfection system specifically designed and optimized for primary keratinocytes. It can be used in the presence of serum and antibiotics and features a simple protocol compatible with both serum-containing and serum-free conditions. The reagent supports multiple culture formats (6-, 12-, 24-, and 96-well plates), and an optimized one-day transfection procedure can be used instead of a conventional two-day workflow, providing time-saving and highly reproducible transfection.
外源DNA导入真核细胞是研究生物学机制最常用的分子生物学技术之一。然而，与转化细胞系相比，原代细胞的高效转染仍具有较大挑战。KeraFectagen是一种基于阳离子聚合物的转染系统，专为原代角质形成细胞设计并优化。该产品可在含血清和抗生素的培养条件下使用，采用简单的操作流程，同时兼容含血清和无血清培养条件。该转染试剂适用于6孔、12孔、24孔和96孔等多种培养板规格，并可采用优化的一天转染方案替代传统的两天转染流程，从而节省实验时间，并实现高效、稳定且具有良好重复性的转染效果。
| 目录编号 | 0963 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | KeraF |
| 规格/数量 | 250 transfections in 96-well plate |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 储存在 -20°C。 |
| 运输 | 干冰。 |