The trophoblast begins at the outer covering of the early blastocyst and provides the route of nourishment between the maternal endometrium and the developing embryo. The trophoblast adhesion to the uterine wall is the requisite first step of implantation and, subsequently, placentation. Human villous tryophoblasts (HVT) covering the villi of the placenta provide the surface for the exchange of oxygen and nutrients with the maternal circulation. They synthesize and release chorionic gonadotropin, placental lactogen and angiogenin and express CXCR4, CCR5 and prolactin gene family. They acquire CCR1 as they differentiate to an invasive phenotype at the villous-anchoring sites. The features of HVT, together with the recent establishment of trophoblast stem cells, make them an ideal genetic platform to study cell differentiation and organogenesis.
HVT from ScienCell Research Laboratories are isolated from human placental villi. HVT are cryopreserved at passage one and delivered frozen. Each vial contains >1 x 106 cells in 1 ml volume. HVT are characterized by HCG ELISA. HVT are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HVT are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HVT are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.
Recommended Medium
It is recommended to use Trophoblast Medium (TM, Cat. #7121) for the culturing of HVT in vitro.
滋养层起源于早期囊胚的外层，为母体子宫内膜与发育中胚胎之间提供营养物质运输途径。滋养层与子宫壁的黏附是胚胎着床以及随后胎盘形成的必要第一步。覆盖胎盘绒毛的人绒毛滋养层细胞（HVT）构成了母体循环与胎盘之间进行氧气和营养物质交换的界面。HVT 可合成并释放绒毛膜促性腺激素、胎盘催乳素和血管生成素，并表达 CXCR4、CCR5 以及催乳素基因家族。HVT 在胎盘绒毛锚定部位向侵袭性表型分化时会获得 CCR1 表达。HVT 的这些特征，加上近年来滋养层干细胞的建立，使其成为研究细胞分化和器官发生的理想遗传学研究平台。
ScienCell Research Laboratories 的 HVT 分离自人胎盘绒毛，于第一代（P1）进行冻存，并以冷冻状态提供。每瓶含有 >1 × 106 个细胞，体积为 1 mL。HVT 通过人绒毛膜促性腺激素（HCG）ELISA 进行表征。HVT 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。在 ScienCell Research Laboratories 提供的培养条件下，HVT 可进一步进行培养；但是，不建议将 HVT 用于细胞扩增或长期培养，因为这些细胞在常规培养条件下不会增殖。
推荐培养基
建议使用滋养层细胞培养基（TM，产品编号 7121）进行 HVT 的体外培养。
Product Sheets
7120
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