人绒毛间充质成纤维细胞

Fibroblasts are mesenchymal cells which are derived from the embryonic mesoderm. Since tissuespecific mesenchymal cells are essential for normal organ development, the villous mesenchymal fibroblasts (VMF) have been used as a model system for studying the cellular mechanisms involved in regulating human placental growth. The VMF lie directly beneath the villous basement membrane; they synthesize HGF, CSF-1, granulocyte CSF and IL-6 and play an important role in the regulation of trophoblast growth and function. Studies have shown that paracrine interaction between placental VMF and the cytotrophoblast in anchoring sites is required for stimulating trophoblast infiltration. HVMF from ScienCell Research Laboratories. 

HVMF from ScienCell Research Laboratories are isolated from human placental villi. HVMF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HVMF are characterized by immunofluorescence with antibodies specific to fibronectin and/or CD90. HVMF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HVMF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HVMF in vitro.

成纤维细胞是来源于胚胎中胚层的间充质细胞。由于组织特异性间充质细胞对正常器官发育至关重要，绒毛间充质成纤维细胞（VMF）已被用作研究调控人胎盘生长的细胞机制模型。VMF 位于绒毛基底膜正下方，可合成肝细胞生长因子（HGF）、集落刺激因子-1（CSF-1）、粒细胞集落刺激因子（G-CSF）和白细胞介素-6（IL-6），并在调控滋养层细胞的生长和功能中发挥重要作用。研究表明，胎盘 VMF 与锚定部位的细胞滋养层细胞之间的旁分泌相互作用，是促进滋养层细胞浸润所必需的。

ScienCell Research Laboratories 的 HVMF 分离自人胎盘绒毛，于第一代（P1）进行冻存，并以冷冻状态提供。每瓶含有 >5 × 10⁵ 个细胞，体积为 1 mL。HVMF 通过免疫荧光法，采用针对纤连蛋白（fibronectin）和/或 CD90 的特异性抗体进行表征。HVMF 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。在 ScienCell Research Laboratories 提供的培养条件下，HVMF 可进一步扩增至少 15 个群体倍增。

推荐培养基

建议使用成纤维细胞培养基（FM，产品编号 2301）进行 HVMF 的体外培养。

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