人源子宫成纤维细胞

During pregnancy, in order to accommodate the growing embryo the uterus undergoes widespread remodeling and dramatic extracellular matrix changes. Studies suggest that activated human uterine fibroblasts (HUF) organize and direct the extracellular matrix remodeling in the uterus throughout pregnancy. Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Uterine fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen. Cultured Human Uterine Fibroblasts (HUF) are a useful in vitro model to study pregnancy and female reproductive disorders.

HUF from ScienCell Research Laboratories are isolated from human uterus. HUF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HUF are characterized by immunofluorescence with antibody specific to fibronectin. HUF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HUF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HUF in vitro.

在妊娠期间，为适应不断生长的胚胎，子宫会发生广泛的组织重塑以及显著的细胞外基质变化。研究表明，活化的人源子宫成纤维细胞（HUF）可在整个妊娠过程中组织并调控子宫细胞外基质的重塑。成纤维细胞是来源于胚胎中胚层的间充质细胞。由于其易于培养，成纤维细胞已被广泛用于各种细胞和分子生物学研究。子宫成纤维细胞可分泌富含Ⅰ型和/或Ⅲ型胶原蛋白的非刚性细胞外基质。培养的人源子宫成纤维细胞（HUF）是研究妊娠过程及女性生殖系统疾病的有价值的体外研究模型。

ScienCell Research Laboratories 提供的人源子宫成纤维细胞（HUF）分离自人源子宫组织。HUF 以第 1 代细胞进行冻存，并以冷冻状态运输。每瓶含有超过 5 x 10⁵ 个细胞，体积为 1 ml。HUF 经采用针对纤连蛋白的特异性抗体进行免疫荧光鉴定。HUF 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。根据 ScienCell Research Laboratories 提供的培养条件，HUF 可保证进一步扩增 15 个群体倍增周期。

推荐培养基

建议使用成纤维细胞培养基（FM，产品编号 #2301）对 HUF 进行体外培养。