Human TRAIL ELISA Kit

In the field of cell biology, TNF-related apoptosis-inducing ligand (TRAIL) is a protein that functions as a ligand to induce the programmed process of cell death, called apoptosis. TRAIL has also been designated CD253 (cluster of differentiation 253). Tumor necrosis factor (TNF) family cytokines function as prominent mediators of immune regulation and inflammatory response. Most TNF family cytokines are expressed as type II transmembrane proteins, with homology confined to approximately 150 C-terminal residues. The TNF ligands interact with a parallel family of receptors. TRAIL binds to the death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII). The process of apoptosis is caspase-8-dependent. Caspase-8 activates downstream effector caspases including procaspase-3, -6, and -7, leading to activation of specific kinases. TRAIL also binds the receptors DcR1 and DcR2, which either do not contain a cytoplasmic domain (DcR1) or contain a truncated death domain (DcR2). The standard product used in this kit is recombinant human TRAIL with a molecular mass of 21 kDa. It is expressed from the amino acids T95-G28.

ScienCell's human TRAIL ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human TRAIL-specific polyclonal antibodies are pre-coated onto 8 x 12 strips. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human TRAIL in the sample that is captured on the strips.