Human Tracheal Fibroblasts

Fibroblasts are mesenchymal cells which perform many vital functions during development and adulthood. Fibroblasts are responsible for the majority of the extracellular matrix synthesis in connective tissue and play major roles in wound healing. In the trachea, fibroblasts and epithelial cells interact in order to respond to tissue injury, bacterial contamination, and other environmental factors. Studies in vitro and in vivo show that tracheal fibroblasts (TrF) can promote tracheal epithelial regeneration by influencing epithelial cell proliferation and differentiation. Abnormal proliferation of Human TrF (HTrF) may lead to the development of airway diseases, such as asthma. HTrF are a useful model for elucidating the mechanisms of wound healing and may provide novel insights for tissue regeneration.

HTrF from ScienCell Research Laboratories are isolated from human trachea. HTrF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HTrF are characterized by their spindle morphology and immunofluorescence with antibodies specific to fibronectin. HTrF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HTrF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for the culturing of HTrF in vitro.