人气管成纤维细胞
人气管成纤维细胞（HTrF）分离自人气管组织。HTrF 以第 1 代（P1）冻存，并以冷冻状态运输。每瓶含有 >5 × 105 个细胞，体积为 1 ml。
Fibroblasts are mesenchymal cells which perform many vital functions during development and adulthood. Fibroblasts are responsible for the majority of the extracellular matrix synthesis in connective tissue and play major roles in wound healing. In the trachea, fibroblasts and epithelial cells interact in order to respond to tissue injury, bacterial contamination, and other environmental factors. Studies in vitro and in vivo show that tracheal fibroblasts (TrF) can promote tracheal epithelial regeneration by influencing epithelial cell proliferation and differentiation. Abnormal proliferation of Human TrF (HTrF) may lead to the development of airway diseases, such as asthma. HTrF are a useful model for elucidating the mechanisms of wound healing and may provide novel insights for tissue regeneration.
HTrF from ScienCell Research Laboratories are isolated from human trachea. HTrF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HTrF are characterized by their spindle morphology and immunofluorescence with antibodies specific to fibronectin. HTrF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HTrF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for the culturing of HTrF in vitro.
成纤维细胞是一类间充质细胞，在胚胎发育和成年期均发挥多种重要的生理功能。成纤维细胞负责结缔组织中大部分细胞外基质的合成，并在伤口愈合过程中发挥重要作用。在气管中，成纤维细胞与上皮细胞相互作用，共同应对组织损伤、细菌污染及其他环境因素。体内和体外研究表明，气管成纤维细胞（TrF）可通过影响上皮细胞的增殖和分化，促进气管上皮的再生。人气管成纤维细胞（HTrF）异常增殖可能导致哮喘等气道疾病的发生。HTrF 可作为研究伤口愈合机制的重要细胞模型，并有助于深入了解组织再生过程。
ScienCell Research Laboratories 的人气管成纤维细胞（HTrF）分离自人气管组织。HTrF 以第 1 代（P1）冻存，并以冷冻状态运输。每瓶含有 >5 × 105 个细胞，体积为 1 ml。HTrF 的鉴定依据包括其梭形细胞形态，以及采用针对纤连蛋白（fibronectin）的特异性抗体进行免疫荧光检测。HTrF 经检测对 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌均呈阴性。在 ScienCell Research Laboratories 提供的培养条件下，HTrF 可进一步扩增 15 个群体倍增（population doublings）。
推荐培养基
推荐使用成纤维细胞培养基（Fibroblast Medium，FM，产品编号：2301）在体外培养 HTrF。
| 目录编号 | 3430 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | HTrF |
| 规格/数量 | 5 x 10^5 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后，立即将细胞从干冰转移到液氮中，并在液氮中保存，直到需要进行实验。 |
| 运输 | 干冰。 |
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