人扁桃体成纤维细胞

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is evidence that fibroblasts in various parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechanical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. Fibroblasts are responsible for much of the synthesis of extracellular matrix in connective tissues and play a major role in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues.

HTF from ScienCell Research Laboratories are isolated from human tonsils. HTF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HTF are characterized by their spindle morphology and immunofluorescence with antibodies specific to fibronectin. HTF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HTF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HTF in vitro.

成纤维细胞是来源于胚胎中胚层的间充质细胞。由于成纤维细胞是较易在体外培养的细胞类型之一，因此被广泛应用于各种细胞和分子生物学研究。其良好的耐受性也使其适用于多种实验操作，包括基因转染和显微注射等研究。研究表明，人体不同部位的成纤维细胞在生物学特性上存在内在差异。组织中的成纤维细胞长期处于动态的机械环境中，这种环境会影响健康组织和损伤修复过程中软组织的结构完整性。成纤维细胞可分泌富含Ⅰ型和/或Ⅲ型胶原蛋白的柔性细胞外基质，在结缔组织细胞外基质的合成以及伤口愈合过程中发挥重要作用。许多疾病都与成纤维细胞有关，其原因可能是成纤维细胞参与了疾病的发生发展，也可能是组织中其他细胞受损后伴随出现的纤维化所致。

ScienCell Research Laboratories 的人扁桃体成纤维细胞（HTF）分离自人扁桃体组织。HTF 以第 1 代（P1）冻存，并以冷冻状态运输。每瓶含有 >5 × 10⁵ 个细胞，体积为 1 ml。HTF 的鉴定依据包括其梭形细胞形态，以及采用针对纤连蛋白（fibronectin）的特异性抗体进行免疫荧光检测。HTF 经检测对 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌均呈阴性。在 ScienCell Research Laboratories 提供的培养条件下，HTF 可进一步扩增 15 个群体倍增（population doublings）。

推荐培养基

推荐使用成纤维细胞培养基（Fibroblast Medium，FM，产品编号：2301）在体外培养 HTF。

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