人源胸腺上皮细胞

Thymic epithelial cells (TyEpiC) are vital regulators of thymocyte development and T lymphocyte (T-cell) tolerance. The thymus contains two types of TyEpiC, cortical and medullary thymic epithelial cells, which regulate the positive selection of thymocytes and the negative selection of autoreactive T-cells. The inability of TyEpiC to remove autoreactive Tcells in the thymus can affect self-tolerance and contribute to the development of autoimmune diseases such as myasthenia gravis, type 1 diabetes, rheumatoid arthritis, and multiple sclerosis. Studies have shown that changes in the expression of tumor necrosis factor receptor family members and transcription factors Foxn1 and autoimmune regulator (Aire) can alter the thymic microenvironment and self-tolerance. Human TyEpiC are an excellent in vitro model to study the mechanisms that modulate TyEpiC functionality and to develop targeted treatments for autoimmune disorders.

HTyEpiC from ScienCell Research Laboratories are isolated from human thymus. HTyEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HTyEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HTyEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HTyEpiC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HTyEpiC are not recommended for expanding or long-term cultures due to limited expansion capacity.

Recommended Medium

It is recommended to use Thymic Epithelial Cell Medium (TyEpiCM, Cat. #3911) for culturing HTyEpiC in vitro.

胸腺上皮细胞（TyEpiC）是调控胸腺细胞发育以及 T 淋巴细胞（T 细胞）耐受性的重要调节细胞。胸腺中包含两类 TyEpiC，即皮质胸腺上皮细胞和髓质胸腺上皮细胞，它们分别调控胸腺细胞的阳性选择以及自身反应性 T 细胞的阴性选择。胸腺中的 TyEpiC 若无法有效清除自身反应性 T 细胞，会影响机体自身耐受性，并促进多种自身免疫性疾病的发生，例如重症肌无力、1 型糖尿病、类风湿性关节炎和多发性硬化症。研究表明，肿瘤坏死因子受体家族成员以及转录因子 Foxn1 和自身免疫调节因子（Aire）的表达变化，可改变胸腺微环境并影响自身耐受性。人源胸腺上皮细胞是研究调控 TyEpiC 功能机制以及开发自身免疫性疾病靶向治疗方法的理想体外研究模型。

ScienCell Research Laboratories 提供的人源胸腺上皮细胞（HTyEpiC）分离自人源胸腺组织。HTyEpiC 以第 1 代细胞进行冻存，并以冷冻状态运输。每瓶含有超过 5 x 10⁵ 个细胞，体积为 1 ml。HTyEpiC 经采用针对细胞角蛋白-18（cytokeratin-18）和/或细胞角蛋白-19（cytokeratin-19）的特异性抗体进行免疫荧光鉴定。HTyEpiC 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。根据 ScienCell Research Laboratories 提供的培养条件，HTyEpiC 可继续进行培养；然而，由于其扩增能力有限，不建议用于细胞扩增或长期培养。

推荐培养基

建议使用胸腺上皮细胞培养基（TyEpiCM，产品编号 #3911）对 HTyEpiC 进行体外培养。