Human Seminal Vesicle Fibroblasts

The seminal vesicles (SV) are a pair of tubular glands located near the prostate and are essential to the urinary system. They function under androgen control to produce and secrete fluid into the ejaculatory duct. The SV consists of three layers: the inner basal mucosal layer composed of simple cuboidal and pseudo-stratified columnar epithelial cells, the middle muscular layer formed by smooth muscle cells, and the outer layer made up of dense connective tissue. Various pathological conditions can arise in the SV, including congenital SV cysts, seminal vesculitis, and primary and secondary neoplasms. Additionally, SV invasion is often used as a prognostic marker in prostate cancer. SV fibroblasts offer unique opportunities to study many features of the SV.

HSVF from ScienCell Research Laboratories are isolated from human seminal vesicle tissue. HSVF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HSVF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HSVF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSVF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for the culturing of HSVF in vitro.