人精囊成纤维细胞
ScienCell Research Laboratories 的人精囊成纤维细胞(HSVF)分离自人精囊组织。HSVF 以第 1 代(P1)冻存,并以冷冻状态运输。每瓶含有 >5 × 105 个细胞,体积为 1 ml。
The seminal vesicles (SV) are a pair of tubular glands located near the prostate and are essential to the urinary system. They function under androgen control to produce and secrete fluid into the ejaculatory duct. The SV consists of three layers: the inner basal mucosal layer composed of simple cuboidal and pseudo-stratified columnar epithelial cells, the middle muscular layer formed by smooth muscle cells, and the outer layer made up of dense connective tissue. Various pathological conditions can arise in the SV, including congenital SV cysts, seminal vesculitis, and primary and secondary neoplasms. Additionally, SV invasion is often used as a prognostic marker in prostate cancer. SV fibroblasts offer unique opportunities to study many features of the SV.
HSVF from ScienCell Research Laboratories are isolated from human seminal vesicle tissue. HSVF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HSVF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HSVF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSVF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for the culturing of HSVF in vitro.
精囊(SV)是一对位于前列腺附近的管状腺体,是男性生殖系统的重要组成部分。在雄激素调控下,精囊负责产生并分泌液体,将其释放至射精管。精囊由三层组织构成:内层为黏膜层,由单层立方上皮细胞和假复层柱状上皮细胞组成;中层为肌肉层,主要由平滑肌细胞构成;外层为致密结缔组织。精囊可发生多种病理性疾病,包括先天性精囊囊肿、精囊炎,以及原发性和继发性肿瘤。此外,精囊受侵常被用作评估前列腺癌预后的指标。人精囊成纤维细胞可为研究精囊的多种生物学特征提供独特的细胞模型。
ScienCell Research Laboratories 的人精囊成纤维细胞(HSVF)分离自人精囊组织。HSVF 以第 1 代(P1)冻存,并以冷冻状态运输。每瓶含有 >5 × 105 个细胞,体积为 1 ml。HSVF 的鉴定依据包括其梭形细胞形态,以及采用针对纤连蛋白(fibronectin)的特异性抗体进行免疫荧光检测。HSVF 经检测对 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌均呈阴性。在 ScienCell Research Laboratories 提供的培养条件下,HSVF 可进一步扩增 15 个群体倍增(population doublings)。
推荐培养基
推荐使用成纤维细胞培养基(Fibroblast Medium,FM,产品编号:2301)在体外培养 HSVF。
| 目录编号 | 4470 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | HSVF |
| 规格/数量 | 5 x 10^5 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准,不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后请立即将细胞从干冰直接转移至液氮中,并在进行细胞培养实验前始终保存在液氮中。 |
| 运输 | 干冰。 |
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