人源胎儿头皮成纤维细胞

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They provide a number of vital functions including synthesizing components of the extracellular matrix, maintaining the structural integrity of connective tissue, and actively participating in wound healing. In addition, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen. Dermal fibroblasts have the ability to switch from a proliferative, migratory phase to a contractile, matrix-remodeling phase during wound healing. Scalp fibroblasts are located in the dermis and play key roles in wound closure. Human Scalp Fibroblasts (HScF) have also been implicated in a number of disorders including androgenetic alopecia and craniosynostosis. HScF can be used to study the pathophysiology of hair loss and craniosynostosis and may help in the development of therapeutics for these diseases.

HScF-f from ScienCell Research Laboratories are isolated from human scalp. HScF-f are cryopreserved at passage one and delivered frozen. Each vial contains > 5 x 10⁵ cells in 1 ml volume. HScF-f are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HScF-f are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HScF-f are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HScF-f in vitro.

成纤维细胞是来源于胚胎中胚层的间充质细胞，具有多种重要生理功能，包括合成细胞外基质成分、维持结缔组织的结构完整性以及积极参与创伤愈合。此外，成纤维细胞还能分泌富含Ⅰ型和/或Ⅲ型胶原蛋白的非刚性细胞外基质。在创伤愈合过程中，真皮成纤维细胞能够由增殖和迁移状态转变为收缩及基质重塑状态。头皮成纤维细胞位于真皮层，在创面闭合过程中发挥关键作用。人源头皮成纤维细胞（HScF）还与多种疾病的发生相关，包括雄激素性脱发和颅缝早闭。HScF 可用于研究脱发和颅缝早闭的病理生理机制，并有助于开发针对这些疾病的治疗方法。

ScienCell Research Laboratories 提供的人源胎儿头皮成纤维细胞（HScF-f）分离自人源头皮组织。HScF-f 以第 1 代细胞进行冻存，并以冷冻状态运输。每瓶含有超过 5 × 10⁵ 个细胞，体积为 1 ml。HScF-f 根据其梭形细胞形态以及采用针对纤连蛋白的特异性抗体进行免疫荧光鉴定。HScF-f 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。根据 ScienCell Research Laboratories 提供的培养条件，HScF-f 可保证进一步扩增 15 个群体倍增周期。

推荐培养基

建议使用成纤维细胞培养基（FM，产品编号 #2301）对 HScF-f 进行体外培养。