肾上皮细胞（REpiC）在肾功能中发挥着关键作用。REpiC 显示出极性形态，这是其功能所必需的。它们几乎能重新吸收肾小球滤液中所有的葡萄糖和氨基酸，同时将其他无营养价值的物质排泄到尿液中。人 REpiC 具有内化细菌的能力。在多种先天性、代谢性和炎症性疾病中，REpiC 位于损伤部位附近。RPTEpiC 可产生炎症介质，如细胞因子或趋化因子，并通过产生 IL-8 影响并引导白细胞趋化，从而积极参与急性炎症过程。REpiC 在炎症期间表达 IL-2R α 和 MHC II 类抗原，表明这些细胞具有参与免疫性肾损伤发病机制的潜力。为研究 REpiC 与多种肾病之间的关系，HRCEpiC 是一个有用的体外模型。
ScienCell Research Laboratories 提供的 HRCEpiC 来源于人肾组织。HRCEpiC 在第一代时冷冻保存，并以冷冻状态交付。每管含有 >5 x 105 个细胞，体积为 1 ml。HRCEpiC 通过针对细胞角蛋白-18 和/或细胞角蛋白-19 的抗体免疫荧光鉴定。HRCEpiC 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母和真菌阴性。在 ScienCell Research Laboratories 提供的条件下，HRCEpiC 保证可继续增殖 15 代。
推荐培养基
建议使用上皮细胞培养基（EpiCM，货号 #4101）对 HRCEpiC 进行体外培养。
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Junctional adhesion molecule-A (JAM-A) is one component of tight junctions which are involved in important processes like paracellular permeability, cell polarity, adhesi... More
Junctional adhesion molecule-A (JAM-A) is one component of tight junctions which are involved in important processes like paracellular permeability, cell polarity, adhesion, migration, and angiogenesis. Here we describe JAM-A expression in distal convoluted tubule, connecting tubule, and in cells of the collecting duct of the healthy human kidney. In addition, JAM-A was weakly expressed in cells of the proximal tubule. Using immunofluorescence, FACS and Western blot analysis we investigated JAM-A expression in tubular cells in vitro. Interestingly, treatment of HK-2 cells with IFN-gamma and TNF-alpha resulted in a metalloproteinase mediated downregulation of JAM-A. Importantly, in a tissue micro-array JAM-A protein expression was significantly downregulated in patients with clear cell renal cell carcinoma. Furthermore, knockdown of JAM-A with JAM-A specific siRNA induced the migration of RCC4 cells. In summary, downregulation of JAM-A is an early event in the development of renal cancer and increases the migration of renal cancer cells. Less
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