人直肠成纤维细胞
分离自人直肠组织。人直肠成纤维细胞（HRecF） 在第一代（P1）阶段进行冻存，并以冷冻形式运输。每瓶含有超过 5 × 105 个细胞，总体积为 1 ml。建议使用成纤维细胞培养基（Fibroblast Medium，FM，产品编号 #2301）对 HRecF 进行体外培养。
The human rectum is located in the lower gastrointestinal tract and functions as a temporary storage site for fecal material. Rectal fibroblasts are important structural components of the rectum and are implicated in a number of diseases including ulcerative colitis, Crohn’s disease, and rectal cancer. Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. The main functions of fibroblasts are to maintain the structural integrity of connective tissue and to aid in tissue repair and remodeling. Studies have shown that functional changes to human rectal fibroblasts (HRecF) can impair wound healing and affect epithelial cell proliferation in ulcerative colitis patients. In Crohn’s disease, HRecF respond to intestinal inflammation by proliferating abnormally, which leads to fibrotic scarring. Primary HRecF can be used to study the pathophysiology of rectal cancer, ulcerative colitis, and Crohn’s disease and to help develop therapies for these diseases.
HRecF from ScienCell Research Laboratories are isolated from human rectum. HRecF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HRecF are characterized by immunofluorescence with antibody specific to fibronectin. HRecF are negative for mycoplasma, bacteria, yeast, and fungi. HRecF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HRecF in vitro.
人直肠位于下消化道，是粪便的暂时储存部位。直肠成纤维细胞是直肠的重要结构组成细胞，与溃疡性结肠炎、克罗恩病及直肠癌等多种疾病密切相关。成纤维细胞是来源于胚胎中胚层的间充质细胞，主要功能包括维持结缔组织的结构完整性，以及参与组织修复与重塑。研究表明，人直肠成纤维细胞（HRecF）的功能变化可能损害伤口愈合，并影响溃疡性结肠炎患者的上皮细胞增殖。在克罗恩病中，HRecF 可对肠道炎症产生响应并发生异常增殖，从而导致纤维化瘢痕形成。原代 HRecF 可用于研究直肠癌、溃疡性结肠炎及克罗恩病的病理生理机制，并为相关疾病的治疗方法开发提供支持。
ScienCell Research Laboratories 提供的人直肠成纤维细胞（HRecF）分离自人直肠组织。HRecF 在第一代（P1）阶段进行冻存，并以冷冻形式运输。每瓶含有超过 5 × 105 个细胞，总体积为 1 ml。HRecF 通过使用针对纤连蛋白（fibronectin）的特异性抗体进行免疫荧光染色鉴定。HRecF 经检测不含支原体、细菌、酵母菌及真菌。在 ScienCell Research Laboratories 提供的培养条件下，HRecF 可保证进一步扩增至少 15 次群体倍增。
推荐培养基
建议使用成纤维细胞培养基（Fibroblast Medium，FM，产品编号 #2301）对 HRecF 进行体外培养。
| 目录编号 | 2960 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | HRecF |
| 规格/数量 | 5 x 10^5 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后请立即将细胞从干冰直接转移至液氮中，并在进行细胞培养实验前始终保存在液氮中。 |
| 运输 | 干冰。 |
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