Human Pulmonary Artery Adventitial Fibroblast cDNA

Human Pulmonary Artery Adventitial Fibroblast PCR-ready first strand cDNA (HPAAF cDNA) is prepared from RNA extracted from Human Pulmonary Artery Adventitial Fibroblasts by a Qiagen RNeasy kit. 2 μg total RNA is then reverse-transcribed using an Applied Biosystems' High-Capacity cDNA Reverse Transcription kit. 1 μl cDNA is sufficient for one PCR reaction. cDNA from ScienCell Research Laboratories is convenient and cost effective for researchers as it eliminates the need to acquire expensive tissues.