成纤维细胞是来源于胚胎中胚层的间充质细胞。由于它们是培养中最容易生长的细胞类型之一，成纤维细胞被广泛用于各种细胞和分子研究。它们的耐久性也使它们适应多种操作，包括基因转染和显微注射等研究。有证据表明，来自不同器官的成纤维细胞本质上是不同的。特别是肺动脉外膜成纤维细胞（PAAF）是血管壁功能的重要调节因子。在病理条件下，如低氧引起的肺动脉高血压，HPAAF变得具有增殖性，促使外膜增厚。
ScienCell研究实验室的HPAAF来源于人类肺动脉。HPAAF在第一级传代时被冷冻保存并交付。每个小瓶包含超过5 x 105个细胞，体积为1毫升。HPAAF具有纺锤形形态，并通过特异性抗纤维连接蛋白的免疫荧光进行表征。HPAAF对HIV-1、HBV、HCV、支原体、细菌、酵母和真菌均为阴性。HPAAF保证在ScienCell研究实验室提供的条件下进一步扩展15个种群倍增。
推荐培养基
建议使用成纤维细胞培养基（FM，Cat. #2301）用于体外培养HPAAF。
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Pulmonary hypertension (PH) arising from chronic lung disease and hypoxia is a deadly condition without cure. Small animal models using hypoxia are mild, and do not fully... More
Pulmonary hypertension (PH) arising from chronic lung disease and hypoxia is a deadly condition without cure. Small animal models using hypoxia are mild, and do not fully recapitulate human disease. The tight skin mouse (Tsk+/−) possesses key characteristics of chronic lung disease, including emphysema. We sought to assess the lung vasculature in Tsk+/− mice exposed to chronic hypoxia. Tsk+/− mice and littermates were housed in normoxia or hypoxia for 4 weeks. Endpoint analysis included invasive measurement of right ventricular systolic pressure, histology, immunoblotting, and ELISA. Proliferation was measured in primary human pulmonary artery adventitial fibroblasts (PAAF) and pulmonary artery smooth muscle cells (PASMC). Tsk+/− mice demonstrated significant emphysema. Following hypoxia, Tsk+/− mice had severe PH as compared to wildtype. Tsk+/− mice had elevated circulating high mobility group box-1 (HMGB1), which was further increased in hypoxia. Treatment with the HMGB1 antagonist, P5779, protected Tsk+/− mice from PH. Tsk+/− lungs displayed increased TGF-β signaling. In vitro, TGF-β stimulated HMGB1 expression in PAAF. HMGB1 in turn stimulated PAAF and PASMC proliferation. Tsk+/− mice represent a robust model of Group 3 PH, driven by hypoxia, sensitized by elevated TGF-β signaling resulting in increased circulating HMGB1, stimulating PAAF and PASMC proliferation. The Tsk+/− model represents a novel system for studying Group 3 PH. Less
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