人脑干神经元

The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain. Despite great variability in size and shape, all neurons share common morphological features, the key elements of a highly complex communication network. Neurons are dynamically polarized cells that serve as the major signaling unit of the nervous system. The brain is known to contain about 1 x 10¹¹ neurons, each being able to contact at least 10,000 other neurons. Brain stem neurons provide main motor and sensory innervation and integrative functions by regulating heart rate, breathing, sleep cycle, awareness, consciousness, and more. Neurons isolated from different brain regions serve as excellent models for studies of region-specific pathologies and degeneration, neurotoxicology and brain development.

HN-bs from ScienCell Research Laboratories are isolated from human brain stem. HN-bs are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. HNbs are characterized by immunofluorescence with antibodies specific to β-tubulin III. HN-bs are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HN-bs are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HN-bs are not recommended for expanding or long-term cultures since the cells do not proliferate in culture

Recommended Medium

It is recommended to use neuronal medium (NM, Cat. #1521) for culturing HN-bs in vitro. 

中枢神经系统组织主要由两大类细胞组成，即神经元和胶质细胞。神经元是大脑的基本解剖、功能和营养单位。尽管神经元在大小和形态上存在很大差异，但它们均具有一些共同的形态学特征，是构成高度复杂神经通信网络的基本要素。神经元是一类具有动态极性的细胞，也是神经系统中负责信号传递的主要功能单位。人脑约含有 1 × 10¹¹ 个神经元，每个神经元至少可与 10,000 个其他神经元建立连接。脑干神经元通过调节心率、呼吸、睡眠周期、觉醒状态、意识等生理功能，为机体提供重要的运动和感觉神经支配，并参与多种整合功能。分离自不同脑区的神经元可作为研究脑区特异性病理和神经退行性变、神经毒理学以及脑发育的理想模型。

ScienCell Research Laboratories 的 HN-bs 分离自人脑干，于 P0 代进行冻存，并以冷冻状态提供。每瓶含有 >1 × 10⁶ 个细胞，体积为 1 mL。HN-bs 通过免疫荧光法，采用针对 β-III 微管蛋白（β-tubulin III）的特异性抗体进行表征。HN-bs 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。在 ScienCell Research Laboratories 提供的培养条件下，HN-bs 可继续进行培养；但是，由于 HN-bs 在培养过程中不具有增殖能力，因此不建议用于扩增培养或长期培养。

推荐培养基

建议使用神经元培养基（NM，产品编号 1521）进行 HN-bs 的体外培养。

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