人源子宫肌层平滑肌细胞

Myometrial smooth muscle cells (MSMC) are located in the myometrium between the endometrium and the serosa of the uterus. MSMC are involved in the stretching of the uterus during pregnancy and are also responsible for uterine contractions during labor. To accommodate the growing embryo, the myometrium expands through smooth muscle cell hypertrophy and hyperplasia. Typically, the uterine myometrium consists of differentiated smooth muscle cells, but in certain pathological conditions, such as uterine leiomyomata, smooth muscle cells in the myometrium proliferate abnormally to form tumors. Studies indicate that platelet-derived growth factor (PDGF) may stimulate MSMC proliferation and consequently may play a role in the formation of leiomyomata. Cultured MSMC are an excellent model to study the physiology of pregnancy and labor and the mechanisms that contribute to the development of uterine leiomyomata.

HMSMC from ScienCell Research Laboratories are isolated from human uterus. HMSMC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HMSMC are characterized by immunofluorescence with antibodies specific to α-smooth muscle actin. HMSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HMSMC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories

Recommended Medium

It is recommended to use Smooth Muscle Cell Medium (SMCM, Cat. #1101) for culturing HMSMC in vitro.

子宫肌层平滑肌细胞（MSMC）位于子宫内膜与浆膜之间的子宫肌层内。MSMC 参与妊娠期间子宫的扩张，并负责分娩过程中的子宫收缩。为了适应不断生长的胚胎，子宫肌层通过平滑肌细胞肥大和增生而扩张。通常情况下，子宫肌层主要由已分化的平滑肌细胞组成；但在某些病理状态下，例如子宫平滑肌瘤，子宫肌层中的平滑肌细胞会发生异常增殖并形成肿瘤。研究表明，血小板衍生生长因子（PDGF）可能刺激 MSMC 增殖，并因此参与子宫平滑肌瘤的形成。培养的人源 MSMC 是研究妊娠和分娩生理过程，以及探究子宫平滑肌瘤发生机制的理想模型。

ScienCell Research Laboratories 提供的人源子宫肌层平滑肌细胞（HMSMC）分离自人源子宫组织。HMSMC 以第 1 代细胞进行冻存，并以冷冻状态运输。每瓶含有超过 5 x 10⁵ 个细胞，体积为 1 ml。HMSMC 经采用针对 α-平滑肌肌动蛋白（α-smooth muscle actin）的特异性抗体进行免疫荧光鉴定。HMSMC 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。根据 ScienCell Research Laboratories 提供的培养条件，HMSMC 可保证进一步扩增 15 个群体倍增周期。

推荐培养基

建议使用平滑肌细胞培养基（SMCM，产品编号 #1101）对 HMSMC 进行体外培养。

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